Lower cadmium accumulation and higher antioxidative capacity in edible parts of Brassica campestris L. seedlings applied with glutathione under cadmium toxicity
Lower cadmium accumulation and higher antioxidative capacity in edible parts of Brassica campestris L. seedlings applied with glutathione under cadmium toxicity
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DOI：
10.1007/s11356-019-04745-7
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发表时间：
2019-05-01
影响因子：
5.8
通讯作者：
Cui, Jin
中科院分区：
文献类型：
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作者：
Huang, Yifan;Zhu, Zhengbo;Cui, Jin
谷胱甘肽（GSH）不仅参与植物的发育过程，而且参与植物对非生物胁迫的应答。采用水培试验研究了外源GSH对镉胁迫下白菜的保护作用。幼苗的形态和生理参数进行分析。结果表明，Cd对水稻生长有严重的抑制作用，并导致水稻植株对Cd的积累。然而，GSH的应用显着减轻镉诱导的毒性症状，包括改善光合作用，植物生长，根系形态学相关参数在镉胁迫下的幼苗。这些反应与代表性的抗氧化酶的活性和相应的非酶抗氧化剂的含量显着增加。O-2（.-）的体内成像脂质过氧化检测进一步证明了GSH提高白菜抗氧化能力的作用。Cd胁迫下，幼苗体内还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）和抗坏血酸/脱氢抗坏血酸（阿萨/DHA）比值显著升高。此外，GSH处理增加了Cd在根中的滞留量，从而显著减少了Cd从根向地上部的转运，最终降低了Cd在地上部的积累。综上所述，我们的研究结果证明GSH通过减少地上部Cd积累和提高抗氧化能力来改善Cd毒害。因此，施用GSH是降低白菜可食部分Cd含量的一种有效途径。在农业生产中。
Glutathione (GSH) is involved in not only plant developmental processes but also plantresponses to abiotic stresses. A hydroponic experiment was performed to explore the protective roles of exogenous GSH in mitigating cadmium (Cd) stress in Brassica campestris L. seedlings by analyzing the morphological and physiological parameters. Results showed that Cd caused severe growth inhibition and Cd accumulation. However, application of GSH significantly mitigated toxic symptoms induced by Cd, including the improvement of the photosynthesis-, plant growth-, and root morphology-related parameters in seedlings under Cd stress. These responses were associated with a striking increase in activities of representative antioxidative enzymes and contents of corresponding non-enzymatic antioxidants. In vivo imaging of O-2(.-) and H2O2, and the detection of lipid peroxidation further demonstrated that increased ability by GSH for Brassica campestris L. seedlings to endure Cd stress was consistent with a striking elevation of ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid to dehydroascorbic acid (AsA/DHA). Additionally, GSH application increased Cd retained in roots, thus significantly decreased its translocation from root to shoot, ultimately decreased Cd accumulation in shoots. Taken together, our results proved evidence for GSH in ameliorating Cd toxicity via reducing Cd accumulation in shoots and increasing oxidation resistance. Accordingly, application of GSH could be a high-efficiency and promising strategy to decrease Cd concentration in edible parts of Brassica campestris L. in agricultural production.