Increase in interleukin (IL)-1 beta and IL-6 in bronchoalveolar lavage fluid obtained from infants with chronic lung disease of prematurity
Increase in interleukin (IL)-1 beta and IL-6 in bronchoalveolar lavage fluid obtained from infants with chronic lung disease of prematurity
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DOI：
10.1203/00006450-199608000-00010
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发表时间：
1996-08-01
影响因子：
3.6
通讯作者：
Shaw, RJ
中科院分区：
文献类型：
--
作者：
Kotecha, S;Wilson, L;Shaw, RJ
早产儿的慢性肺病(CLD)与最初的肺中性粒细胞增加以及随后的肺纤维化有关。我们检测了9例慢性阻塞性肺疾病(CLD)患儿、7例呼吸窘迫综合征(RDS)恢复期患儿和4例对照组(38wk和2690g)新生儿的支气管肺泡灌洗液中促炎症细胞因子IL-1β和IL-6是否升高。与RDS组和对照组比较，CLD组肺泡灌洗液中IL-1β和n-6蛋白均升高。当比较患有和不患有CLD的婴儿的结果时，这两种细胞因子的差异在第10天最显著(IL-1β，4.6对1.1 ng/m L，p<；0.05；n-6，9.5对1.5 ng/m l，p<；0.05)。IL-1β、IL-6和IL-8蛋白免疫细胞化学显示肺泡巨噬细胞含有这三种细胞因子，中性粒细胞和偶见灌洗获得的上皮细胞染色较弱。肺泡巨噬细胞和腔细胞在IL-6和IL-1升高中的作用通过对灌洗液细胞提取的RNA进行半定量逆转录-聚合酶链式反应来确定。与RDS组比较，CLD患儿灌洗液细胞IL-6mRNA表达增加。然而，IL-1β和IL-8在两组中的表达相似。这些结果提示，IL-1β、IL-6和IL-8可能参与了CLD的发病，而在CLD中，n-6可能是由空气中的细胞产生的。
Chronic lung disease (CLD) of prematurity is associated with an initial increase in pulmonary neutrophils followed by pulmonary fibrosis. We determined whether the proinflammatory cytokines, IL-1 beta and IL-6, were increased in the bronchoalveolar lavage fluid obtained from nine infants (median gestation 25 wk, birthweight 820 g) who developed CLD, seven (28 wk, 1110 g) who recovered from the respiratory distress syndrome (RDS), and four (38 wk, 2690 g) control infants. IL-1 beta and n-6 protein were both increased in the bronchoalveolar lavage fluid from the CLD groups when compared with the RDS and control groups. This difference for both the cytokines was most marked on d 10 of age, when results from infants with and without CLD were compared (IL-1 beta, 4.6 versus 1.1 ng/mL, p < 0.05; and n-6, 9.5 versus 1.5 ng/mL, p < 0.05). Immunocytochemistry of lavage cells for IL-1 beta, IL-6, and IL-8 protein showed alveolar macrophages to contain all three cytokines, with lesser staining evident in neutrophils, and in epithelial cells occasionally obtained by lavage. The contribution of alveolar macrophages and luminal cells to the increase in IL-6 and IL-1 was determined by per forming semiquantitative reverse transcription-polymerase chain reactions on RNA extracted from lavage cells. IL-6 mRNA expression was increased in lavage cells from the CLD infants when compared with the RDS group. However, the expression for IL-1 beta and IL-8 mRNA was similar in both groups. These results suggest that IL-1 beta, IL-6, and IL-8 may contribute to the pathogenesis of CLD, and that, in CLD, n-6 may be produced by cells within the air spaces.