Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter
Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter
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DOI：
10.1126/science.289.5481.957
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发表时间：
2000-08-11
期刊：
影响因子：
56.9
通讯作者：
Edwards, RH
中科院分区：
文献类型：
--
作者：
Bellocchio, EE;Reimer, RJ;Edwards, RH
以前的工作已经确定了两个家族的蛋白质，运输经典的神经递质进入突触囊泡，但负责囊泡运输的主要兴奋性递质谷氨酸的蛋白质仍然未知。我们证明了一种与任何已知的神经递质转运蛋白无关的蛋白质，该蛋白质先前被建议介导跨质膜的无机磷酸盐的Na+依赖性摄取，将谷氨酸转运到突触囊泡中。此外，我们表明，这种囊泡谷氨酸转运蛋白，VGLUT1，表现出的电导氯被谷氨酸阻断。
Previous work has identified two families of proteins that transport classical neurotransmitters into synaptic vesicles, but the protein responsible for vesicular transport of the principal excitatory transmitter glutamate has remained unknown. We demonstrate that a protein that is unrelated to any known neurotransmitter transporters and that was previously suggested to mediate the Na+-dependent uptake of inorganic phosphate across the plasma membrane transports glutamate into synaptic vesicles. In addition, we show that this vesicular glutamate transporter, VGLUT1, exhibits a conductance for chloride that is blocked by glutamate.