Dietary fish oil inhibits antigen-specific murine Th1 cell development by suppression of clonal expansion.
Dietary fish oil inhibits antigen-specific murine Th1 cell development by suppression of clonal expansion.
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DOI：
10.1093/jn/136.9.2391
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发表时间：
2006-09
期刊：
影响因子：
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通讯作者：
Ping Zhang;Wooki Kim;Lan Zhou;Naisyin Wang;L. Ly;D. Mcmurray;R. Chapkin
中科院分区：
文献类型：
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作者：
Ping Zhang;Wooki Kim;Lan Zhou;Naisyin Wang;L. Ly;D. Mcmurray;R. Chapkin
为了确定膳食鱼油（FO）影响抗原刺激的Th 1细胞发育的机制，给DO11.10 Rag 2（-/-）T细胞受体转基因小鼠喂食对照饮食（5%玉米油（CO））或FO饮食（1% CO + 4% FO，（n-3）PUFA）2周。在中性或Th 1极化条件下培养CD 4（+）T细胞。FO喂养抑制（P < 0.05）卵清蛋白肽诱导的非极化CD 4（+）T细胞增殖。体外向Th 1细胞的分化不受饮食FO的影响，如来自CO喂养（99%）和FO喂养（97%）的小鼠的培养物中KJ 1 -26（+）、IFN-γ（+）、IL-4（-）Th 1细胞的相似百分比所证明的。然而，FO喂养小鼠极化培养物中活Th 1细胞的绝对数量不到CO喂养小鼠中观察到的一半（P < 0.05），表明FO抑制体外Th 1克隆扩增。FO培养物中Th 1细胞数量的减少不是凋亡增加的结果，因为在FO和CO喂养的小鼠培养物中观察到凋亡Th 1细胞的相似百分比。FO组极化Th 1细胞中IL-2诱导的细胞增殖显著降低;然而，增殖抑制与抗原刺激的CD 4（+）T细胞上CD 25表面表达降低无关。将CFSE标记的DO11.10 CD 4（+）细胞连续转移到免疫小鼠（Th 1极化剂）体内，结果表明，饲料FO降低了体内细胞分裂数（P < 0.05）。这些研究表明，衰减的炎症反应，伴随着FO喂养可以解释，至少部分，抑制Th 1克隆扩增。
To determine the mechanisms by which dietary fish oil (FO) affects antigen-stimulated Th1 cell development, DO11.10 Rag 2(-/-) T cell receptor transgenic mice were fed a control diet (5% corn oil (CO) or a FO diet (1% CO + 4% FO, (n-3) PUFA) for 2 wk. CD4(+) T cells were cultured under neutral or Th1 polarizing conditions. FO feeding suppressed (P < 0.05) ovalbumin peptide-induced proliferation of nonpolarized CD4(+) T cells. Differentiation in vitro to Th1 cells was not affected by dietary FO, as evidenced by similar percentages of KJ1-26(+), IFN-gamma(+), IL-4(-) Th1 cells in cultures from CO-fed (99%) and FO-fed (97%) mice. However, the absolute number of viable Th1 cells in polarized cultures from FO-fed mice was less than half that observed in CO-fed mice (P < 0.05), indicating that FO inhibits in vitro Th1 clonal expansion. The reduced number of Th1 cells in FO cultures was not a result of increased apoptosis, because similar percentages of apoptotic Th1 cells were observed in cultures from FO- and CO-fed mice. IL-2-induced cell proliferation was significantly decreased in polarized Th1 cells from the FO group; however, the suppressed proliferation was not linked to reduced CD25 surface expression on antigen-stimulated CD4(+) T cells. Adoptively transferred CFSE-labeled DO11.10 CD4(+) cells into immunized mice (Th1 polarizing agents) showed that dietary FO reduced (P < 0.05) the number of cell divisions in vivo. These studies suggest that the attenuated inflammatory response which accompanies FO feeding may be explained, at least in part, by suppression of Th1 clonal expansion.