Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides.
Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides.
复制标题
DOI：
10.1021/acsami.0c21760
复制
发表时间：
2021-01
影响因子：
9.5
通讯作者：
Yonghong Zha;Shi-Ying Lu;P. Hu;H. Ren;Zengshan Liu;Weihua Gao;Chengmin Zhao;Yansong Li;Yu Zhou
中科院分区：
文献类型：
--
作者：
Yonghong Zha;Shi-Ying Lu;P. Hu;H. Ren;Zengshan Liu;Weihua Gao;Chengmin Zhao;Yansong Li;Yu Zhou
卫生保健和环境监测需要方便和超灵敏的农药检测，这可以通过双模式策略来实现。基于生物素标记的IgG修饰金纳米粒子（AuNP@IgG-bio）探针，构建了一种检测氯乙酰胺类除草剂的双模态免疫传感器。该平台依赖于碱性磷酸酶（ALP）对2-磷酸抗坏血酸（AA 2 P）的去磷酸化。除了这个过程，抗坏血酸（AA）触发的沉积银上的金纳米星（AuNS）和脱氢AA和邻苯二胺（OPD）的荧光反应依次发生。因此，可以使用由晶体生长诱导的红-绿-蓝（RGB）的颜色变化和荧光生成的双重读出。智能手机的检测限（LOD）低至1.20 ng/mL乙草胺（ATC）、0.89 ng/mL异丙甲草胺、1.22 ng/mL异丙草胺和0.99 ng/mL它们的混合物，荧光分光光度计的检测限（LOD）低至0.44 ng/mL ATC、1.59 ng/mL异丙甲草胺、2.80 ng/mL异丙草胺和0.72 ng/mL它们的混合物。玉米样品的回收率为比色法的91.4-105.1%，荧光法的92.4-106.2%。该双模态免疫传感器具有观察方式简单、性能优良等优点，具有广阔的应用前景。
Convenient and ultrasensitive detection of pesticides is demanded for healthcare and environmental monitoring, which can be realized with a dual-modal strategy. In this paper, based on a biotin-labeled IgG-modified gold nanoparticle (AuNP@IgG-bio) probe, a dual-modal immunosensor was proposed for detecting chloroacetamide herbicides. This platform is relied on the dephosphorylation of ascorbic acid 2-phosphate (AA2P) by alkaline phosphatase (ALP). In addition to this process, ascorbic acid (AA)-triggered deposition of silver on gold nanostars (AuNSs) and the fluorogenic reaction of dehydrogenated AA and o-phenylenediamine (OPD) occur sequentially. Thus, the dual readout of the color change of red-green-blue (RGB) and fluorescence generation in situ induced by crystal growth can be used. The limits of detection (LODs) were as low as 1.20 ng/mL of acetochlor (ATC), 0.89 ng/mL of metolachlor, 1.22 ng/mL of propisochlor, and 0.99 ng/mL of their mixture by a smartphone and 0.44 ng/mL of ATC, 1.59 ng/mL of metolachlor, 2.80 ng/mL of propisochlor, and 0.72 ng/mL of their mixture by a spectrofluorometer. The recoveries from corn were 91.4-105.1% of the colorimetric mode and 92.4-106.2% of the fluorescent mode. Due to its simple observation mode and good performance, this dual-modal immunosensor possesses considerable application prospects.