Biologic and molecular genetic characteristics of a unique MCF virus that is highly leukemogenic in ecotropic virus-negative mice.
Biologic and molecular genetic characteristics of a unique MCF virus that is highly leukemogenic in ecotropic virus-negative mice.
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一种独特的 MCF 病毒的生物学和分子遗传特征，该病毒在亲嗜性病毒阴性小鼠中具有高度致白血病性。
DOI：
10.1016/0042-6822(89)90407-8
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发表时间：
1989
期刊：
影响因子：
3.7
通讯作者：
Hartley,JW
中科院分区：
文献类型：
--
作者：
Chattopadhyay,SK;Baroudy,BM;Holmes,KL;Fredrickson,TN;Lander,MR;Morse3rd,HC;Hartley,JW
加州野生小鼠衍生的亲嗜性病毒 Cas-Br-M 在接种新生小鼠后可诱发海绵状脑病和多种造血系统肿瘤。我们从接种 Cas-Br-M 病毒的 NFS 小鼠中产生的胸腺 T 细胞淋巴瘤中分离出 MCF 病毒 [NS-6(186) MCF]。与之前研究的MCF病毒相比，生物克隆的NS-6(186) MCF病毒被发现在没有亲嗜性辅助病毒的情况下能够高效诱导胸腺或非胸腺T细胞淋巴瘤。比较 Cas-Br-M 和 NS-6(186) MCF 的限制性内切酶图谱发现，差异仅存在于 env 区域，距 5' 端 5.8 至 7.8 kb 之间。研究了 NS-6(186) MCF 的两个生物活性分子克隆（具有两个 LTR 的克隆 15 和具有 1 个 LTR 的克隆 19）。尽管两个克隆表现出相似的体外活性，但克隆 15 衍生的病毒仅诱导 T 细胞淋巴瘤，潜伏期较短，而克隆 19 衍生的病毒则诱导多种肿瘤，潜伏期明显较长。核苷酸序列分析确定，克隆 15 的两个 LTR 中的每一个的 U3 区域都有一个 53 bp 的重复，其中包括“增强子元件”，但克隆 19 的单个 LTR 没有这样的重复。
California wild mouse-derived ecotropic virus Cas-Br-M induces a spongiform encephalopathy and a wide variety of hematopoietic neoplasms on inoculation of neonatal mice. We isolated a MCF virus [NS-6(186) MCF] from a thymic T-cell lymphoma developing in a NFS mouse inoculated with Cas-Br-M virus. Biologically cloned NS-6(186) MCF virus, in contrast to previously studied MCF viruses, was found to induce thymic or nonthymic T-cell lymphomas with high efficiency in the absence of ecotropic helper virus. Comparison of the restriction endonuclease maps derived from Cas-Br-M and NS-6(186) MCF revealed differences only in the env region, between 5.8 and 7.8 kb from the 5′ end. Two biologically active molecular clones of the NS-6(186) MCF (clone 15 with two LTRs and clone 19 with 1 LTR) were studied. Although both clones exhibited similarin vitroactivities, clone 15-derived virus induced only T-cell lymphomas with short latency whereas clone 19-derived virus induced a wide variety of neoplasms with a significantly longer latency. Nucleotide sequence analysis established that the U3 region of each of the two LTRs of clone 15 has a 53-bp duplication which includes “enhancer elements,” but that the single LTR of clone 19 has no such duplication.