Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers
Towards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers
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DOI：
10.1007/s00438-002-0665-6
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发表时间：
2002-05-01
影响因子：
3.1
通讯作者：
Grattapaglia, D
中科院分区：
文献类型：
--
作者：
Brondani, RPV;Brondani, C;Grattapaglia, D
在现有的巨桉（Eucalyptus grandis）和巨桉（Eucalyptus grandis）的RAPD框架图上，构建了一套50个高度多态的微卫星标记。尾叶这些标记与之前开发的二十个微卫星标记一起，用于对齐热带地区两种最具商业重要性的桉树物种的现有地图。将63个微卫星标记定位在E. grandis在11个连锁群中定位，E. urophylla图谱分布在10个连锁群中。大约66%的微卫星标记分离在一个完全信息丰富的方式，允许建立共线同线连锁群之间的两个地图。这50个新的微卫星标记信息量很大，平均每个位点有14个等位基因，平均期望杂合度在0.82 ~ 0.87之间。此外，在Symphyomyrtus亚属内。绝大多数商业上重要的桉树属种都属于这种类型，这些标记物显示出平均90%的可运输性。这组70个微卫星标记的映射是一个重要的一步，为桉树全属参考连锁图谱的发展。这些高度多等位基因和可迁移的标记构成了QTL发现和验证的有力工具，并可用于在桉树家系中的QTL等位基因变异的定向搜索。
A novel set of 50 highly polymorphic microsatellite markers were developed and mapped on existing RAPD framework maps of Eucalyptus grandis and E. urophylla. Together with the twenty previously developed microsatellite markers, these were used to align the existing maps for the two most commercially important Eucalyptus species in the tropics. Sixty-three microsatellite markers were placed on the E. grandis map in 11 linkage groups, and 53 on the E. urophylla map distributed in 10 linkage groups. Approximately 66% of the microsatellite markers segregated in a fully informative fashion, allowing the establishment of colinear syntenic linkage groups between the two maps. The 50 new microsatellite markers were highly informative, with an average of 14 alleles per locus, and average expected heterozygosity between 0.82 and 0.87. Furthermore, within the subgenus Symphyomyrtus. to which the vast majority of commercially important Eucalyptus species belong, these markers display on average 90% transportability. This set of 70 mapped microsatellite markers represents a significant step toward the development of a genus-wide reference linkage map for Eucalyptus. These highly multiallelic and transportable markers constitute a powerful tool for QTL discovery and validation, and can be used in directed searches for QTL allele variation across Eucalyptus pedigrees.