IMMUNOHISTOCHEMICAL INVESTIGATION OF ISCHEMIC AND POSTISCHEMIC DAMAGE AFTER BILATERAL CAROTID OCCLUSION IN GERBILS
IMMUNOHISTOCHEMICAL INVESTIGATION OF ISCHEMIC AND POSTISCHEMIC DAMAGE AFTER BILATERAL CAROTID OCCLUSION IN GERBILS
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DOI：
10.1161/01.str.19.12.1526
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发表时间：
1988-12-01
期刊：
影响因子：
8.3
通讯作者：
YANAGIHARA, T
中科院分区：
文献类型：
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作者：
HATAKEYAMA, T;MATSUMOTO, M;YANAGIHARA, T
采用免疫组织化学方法（微管蛋白和肌酸激酶bb -同工酶的反应）研究了双侧颈总动脉闭塞后沙鼠全脑缺血期间和之后神经元损伤的进展和恢复情况。最早但可逆的缺血性病变发生在海马丘下- ca1和CA2区缺血3分钟后。缺血4分钟后，病变变得不可逆。如果缺血期为5分钟，大脑皮层、丘脑和尾核的缺血和缺血后病变部分或完全可逆，而再灌注48小时后，内侧CA1区锥体细胞发生迟发性变性（迟发性神经元死亡）。缺血再灌注10分钟后，延迟性神经元死亡从CA1区内侧延伸到外侧；再灌注时，大脑皮层、丘脑和尾核的缺血和缺血后病变也扩大。我们的研究表明，选择性易损在全脑缺血和不完全或局部缺血中都存在，并表明大脑许多区域的神经元具有恢复、进行性退化甚至延迟神经元死亡的潜力，这取决于脑缺血的严重程度和持续时间。
We investigated progression and recovery of neuronal damage during and after global cerebral ischemia in gerbils after bilateral occlusion of the common carotid arteries, using the immunohistochemical method (reaction for tubulin and creatine kinase BB-isoenzyme). The earliest, but reversible, ischemic lesions occurred after 3 minutes'' ischemia in the subiculum-CA1 and CA2 regions of the hippocampus. The lesions became irreversible after 4 minutes'' ischemia. The ischemic and postischemic lesions in the cerebral cortex, thalamus, and caudoputamen were partially or completely reversible if the ischemic period was 5 minutes, whereas delayed degeneration occurred in the pyramidal cells of the medial CA1 region after reperfusion for 48 hours (delayed neuronal death). After 10 minutes'' ischemia and subsequent reperfusion, delayed neuronal death extended from the medial to the lateral CA1 region; the ischemic and postischemic lesions in the cerebral cortex, thalamus, and caudoputamen also expanded during reperfusion. Our investigation demonstrates that selective vulnerability existed in global cerebral ischemia as in incomplete or regional ischemia and suggests that neurons in many areas of the brain possessed the potential for recovery, progressive deterioration, and even delayed neuronal death depending on the severity and duration of cerebral ischemia.