Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide
Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide
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里氏木霉的比较转录组分析揭示了葡萄糖和β-二糖的合成混合物诱导的不同基因调控网络
DOI：
10.1186/s40643-021-00411-4
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发表时间：
2021-07-03
影响因子：
4.6
通讯作者：
Li, Qian
中科院分区：
文献类型：
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作者：
Li, Yonghao;Yu, Jingze;Li, Qian
由葡萄糖转糖基作用合成的葡萄糖和β-二糖混合物（MGD）作为一种低成本的可溶性碳源，能有效诱导里氏木霉产生纤维素酶，这在木质纤维素生物质的生物精炼方面具有潜力。然而，MGD如何诱导里氏木霉纤维素酶产生尚未完全清楚。在本研究中，通过转录组分析来研究与乳糖相比，MGD诱导里氏木霉Rut C30产生木质纤维素降解酶的分子基础。特别关注了与木质纤维素降解相关的碳水化合物活性酶（CAZymes）、转录因子、转运蛋白以及其他蛋白质加工途径。结果表明，MGD能诱导编码GH5、GH6和GH7的纤维素酶转录，其水平比乳糖诱导的高1.4倍，但编码GH11和GH74的木聚糖酶分别下调了1.7倍和4.4倍。基因表达谱显示，与乳糖诱导的情况相比，转录激活因子xyr1和vib1显著上调，丝裂原活化蛋白激酶途径增强。此外，MGD显著上调编码未折叠蛋白反应（UPR）特异性转录因子的hac1，这可能是由于新生蛋白质的正确折叠和加工得到增强。这些发现为进一步了解在里氏木霉中使用MGD作为诱导剂高效产生纤维素酶的特性提供了理论基础，并为菌株改良提供了潜在策略。
The mixture of glucose and beta-disaccharide (MGD) synthesized by transglycosylation of glucose as a low-cost soluble carbon source can efficiently induce cellulase production in Trichoderma reesei, which holds potential for the biorefining of lignocellulosic biomass. However, it is not yet fully understood how MGD induces T. reesei cellulase. In this study, transcriptomic analyses were conducted to investigate the molecular basis of MGD for lignocellulose-degrading enzyme production of T. reesei Rut C30 compared with that on lactose. Particular attention was paid to CAZymes, transcription factors, transporters and other protein processing pathways related to lignocellulose degradation. As a result, MGD can elicit transcription of GH5-, GH6- and GH7-encoding cellulases that is up to 1.4-fold higher than that induced by lactose, but GH11- and GH74-encoding xylanases are downregulated by 1.7- and 4.4-fold, respectively. Gene expression profiles suggest that the transcription activators xyr1 and vib1 are significantly upregulated and that the mitogen-activated protein kinase pathway is strengthened compared to the case of lactose induction. In addition, hac1-encoding UPR-specific transcription factors are significantly upregulated by MGD, which may be enhanced due to proper folding and processing of nascent proteins. These findings provide a theoretical basis for further understanding the characterization of efficient cellulase production using MGD as an inducer in T. reesei and offer potential strategies for strain improvement.