Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton
Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton
复制标题
棉花CMS-D8和CMS-D2育性恢复基因InDel标记的开发和利用
DOI：
10.1007/s11033-019-05240-5
复制
发表时间：
2020-02-01
影响因子：
2.8
通讯作者：
Wu, Jianyong
中科院分区：
文献类型：
--
作者：
Feng, Juanjuan;Zhu, Haiyong;Wu, Jianyong
The cytoplasmic male sterility (CMS) system is a useful tool for commercial hybrid cotton seed production. Two main CMS systems, CMS-D8 and CMS-D2, have been recognized withRf2andRf1as the restorer genes, respectively. The development of molecular markers tightly linked with restorer genes can facilitate the breeding of restorer lines. In this study, the InDel-1892 marker was developed to distinguishRf2andRf1simultaneously. Sequence alignment implied that CMS-D8-Rf2has a 32 bp insertion and that CMS-D2-Rf1has a 186 bp insertion at the InDel-1892 locus. The codominant marker was co-segregated withRf1andRf2. Hence, this marker can be used for tracingRf1andRf2simultaneously and identifying the allele status at the restorer gene locus. The results of this study will facilitate efficient marker-assisted selection for restorer lines and hybrids of CMS systems.
