Evaluation of the internal colonization of Atriplex canescens (Pursh) Nutt. roots by dark septate fungi and the influence of host physiological activity
Evaluation of the internal colonization of Atriplex canescens (Pursh) Nutt. roots by dark septate fungi and the influence of host physiological activity
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DOI：
10.1007/s005720100111
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发表时间：
2001-09-01
期刊：
影响因子：
3.9
通讯作者：
Aaltonen, RE
中科院分区：
文献类型：
--
作者：
Barrow, JR;Aaltonen, RE
暗隔（DS）真菌内生菌是四翼滨藜，四翼滨藜（Pursh）Nutt，在美国西南部牧场的优势和生态重要的灌木的主要根殖民者。这些真菌的特征是使用常规真菌染色方法在根皮层中鉴定为染色或着色的菌丝和微菌核。A.用苏丹红IV染色DS真菌定殖的canescens根，并用微分干涉显微镜分析。该方法揭示了大量的内部殖民的皮质和维管柱空泡化的透明菌丝，是不明显的台盼蓝染色时。透明菌丝是黑化的DS菌丝和微菌核的内部延伸。苏丹红IV强烈染色脂质在真菌空泡，进一步提高能见度的内部透明菌丝。黑化的菌丝和微菌核更丰富的根取样休眠和相对不活跃的植物，而透明的菌丝和脂质积累是最普遍的生理活性植物的根。DS真菌内生菌的多态性，它们对代谢活动的动态响应，以及它们与A.根进行了讨论。
Dark septate (DS) fungal endophytes are the primary root colonizers of fourwing saltbush, Atriplex canescens (Pursh) Nutt, a dominant and ecologically important shrub in southwestern USA rangelands. These fungi are characteristically identified as stained or pigmented hyphae and microsclerotia in the root cortex using conventional fungus staining methods. A. canescens roots colonized by DS fungi were stained with sudan IV and analyzed with differential interference microscopy. This method revealed substantial internal colonization of the cortex and vascular cylinder by vacuolated hyaline hyphae that were not evident when stained with trypan blue. Hyaline hyphae were internal extensions of melanized DS hyphae and microsclerotia. Sudan IV intensely stained lipids in fungal vacuoles, further enhancing visibility of internal hyaline hyphae. Melanized hyphae and microsclerotia were more abundant in roots sampled from dormant and relatively inactive plants, while hyaline hyphae and lipid accumulation were most prevalent in roots of physiologically active plants. The polymorphic nature of DS fungal endophytes, their dynamic response to metabolic activity, and their similarities and differences relative to aseptate fungal colonization in A. canescens roots are discussed.