INVIVO TRANSFECTION OF MURINE LUNGS WITH A FUNCTIONING PROKARYOTIC GENE USING A LIPOSOME VEHICLE
INVIVO TRANSFECTION OF MURINE LUNGS WITH A FUNCTIONING PROKARYOTIC GENE USING A LIPOSOME VEHICLE
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DOI：
10.1097/00000441-198910000-00013
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发表时间：
1989-10-01
影响因子：
3.1
通讯作者：
BERRY, LC
中科院分区：
文献类型：
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作者：
BRIGHAM, KL;MEYRICK, B;BERRY, LC
作者报道了用编码细胞内酶氯霉素乙酰转移酶（CAT）的功能性原核基因成功地体内转染小鼠肺。通过注射含有CAT编码区的质粒完成转染，所述CAT编码区由与专门合成的阳离子脂质体复合的SV40早期启动子（pSV2CAT）驱动。静脉或肺内注射DNA脂质体导致CAT基因在肺中表达，持续至少一周，在全身器官中几乎检测不到酶活性。该方法应允许用编码任何感兴趣的蛋白质的基因瞬时或稳定地体内转染肺，提供了强大的实验工具和潜在的新的和广泛适用的临床治疗技术。
The authors report successful in vivo transfection of lungs of mice with a functioning prokaryotic gene encoding the intracellular enzyme, chloramphenicol acetyltransferase (CAT). Transfection was accomplished by injecting a plasmid containing the coding region for CAT driven by the SV40 early promoter (pSV2CAT) complexed to specially synthesized cationic liposomes. Intravenous or intratracheal injection of DNA-liposomes resulted in expression of the CAT gene in the lungs, persisting for at least a week, with little enzyme activity detectable in systemic organs. This method should permit either transient or stable in vivo transfection of the lungs with a gene encoding any protein of interest, providing a powerful experimental tool and potentially a novel and broadly applicable clinical therapeutic technique.