Mechanisms of Dendritic Elaboration of Sensory Neurons in Drosophila: Insights from In Vivo Time Lapse
Mechanisms of Dendritic Elaboration of Sensory Neurons in Drosophila: Insights from In Vivo Time Lapse
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DOI：
10.1523/jneurosci.4521-03.2004
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发表时间：
2004-02
期刊：
影响因子：
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通讯作者：
Darren W. Williams;J. Truman
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文献类型：
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作者：
Darren W. Williams;J. Truman
在体内延时多光子显微镜被用来分析的树突状分支（da）的感觉神经元称为背树突状分支神经元E（ddaE）在变态过程中的重塑。在其幼虫过程已被删除后，ddaE的细胞体重新定位自己的体壁之间的25和40小时后，蛹形成（APF）和40小时APF开始其成人的生长。乔木的支架是奠定了40和54小时APF之间，当生长的特点是高丝状伪足活动在终端和间隙位置和分支收缩沿着分支建立。在发育后期，丝状伪足活动仍然很高，但仅限于终末分支，分支回缩不再可见。昆虫激素保幼激素（JH），变态的一个关键调节器，治疗改变了成年树突树的形状和复杂性在一个时间依赖性的方式。早期治疗与保幼激素模拟物（JHm）似乎抑制延伸程序和保持收回程序。在以后的JHm治疗中，伸展程序似乎正常，但收缩程序保持超过正常时间。JH处理显示了回缩程序在建立整体刀杆形状中的重要性。
In vivo time-lapse multiphoton microscopy was used to analyze the remodeling of the dendritic arborizing (da) sensory neuron known as dorsal dendritic arborizing neuron E (ddaE) during metamorphosis. After its larval processes have been removed, the cell body of ddaE repositions itself on the body wall between 25 and 40 hr after puparium formation (APF) and begins its adult outgrowth at 40 hr APF. The scaffold of the arbor is laid down between 40 and 54 hr APF, when growth is characterized by high filopodial activity at both terminal and interstitial positions and by branch retraction along with branch establishment. Later in development, filopodial activity remains high but is confined to terminal branches, and branch retraction is no longer seen. Treatment with the insect hormone juvenile hormone (JH), a key regulator of metamorphosis, alters the shape and complexity of the adult dendritic tree in a time-dependent manner. Early treatments with juvenile hormone mimic (JHm) appear to repress extension programs and maintain retraction programs. With later JHm treatments, extension programs appear normal, but retraction programs are maintained beyond their normal time. The JH treatments show the importance of retraction programs in establishing the overall arbor shape.