FORMAMIDINES INTERACT WITH DROSOPHILA OCTOPAMINE RECEPTORS, ALTER THE FLIES BEHAVIOR AND REDUCE THEIR LEARNING-ABILITY
FORMAMIDINES INTERACT WITH DROSOPHILA OCTOPAMINE RECEPTORS, ALTER THE FLIES BEHAVIOR AND REDUCE THEIR LEARNING-ABILITY
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DOI：
10.1007/bf00605015
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
OFARIM, M
中科院分区：
文献类型：
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作者：
DUDAI, Y;BUXBAUM, J;OFARIM, M
本文研究了甲脒对果蝇的影响。低浓度的甲脒对果蝇成虫有毒性。cAMP合成减少的突变体显示出对毒素的抗性增加。甲脒还能降低果蝇卵的存活率，延缓成虫羽化。在亚致死浓度下，甲脒明显影响苍蝇的行为。注射后，化合物增加肌肉活动。在喂食时，甲脒诱导运动兴奋，减少趋光性和损害嗅觉学习，而不影响识别嗅觉线索的能力。在体外，两个甲脒被发现抑制章鱼胺刺激的腺苷酸环化酶，而不影响酶的基础活性，而第三个被发现刺激腺苷酸环化酶，这种刺激被阻断酚妥拉明和在较小程度上由普萘洛尔，从而类似的效果章鱼胺。[~ 3 H]章鱼胺与果蝇前膜的结合也受到抑制。两者合计，我们的研究结果表明，甲脒相互作用与章鱼胺能系统在果蝇，发挥外周和中央的影响，在苍蝇，并可用于解剖章鱼胺在发展和行为，包括行为可塑性的作用。结果还表明，甲脒可用于选择胺能传递和cAMP级联中的突变体。
We have studied the effect of formamidines onDrosophila melanogaster. Low concentrations of formamidines are toxic to adultDrosophila. A mutant with reduced cAMP synthesis displays increased resistance to the toxin. Formamidines also reduce viability ofDrosophilaeggs and retard imago eclosion. At sublethal concentrations, formamidines markedly affect the flies' behavior. Upon injection, the compounds increase muscle activity. Upon feeding, formamidines induce motor excitation, reduce phototaxis and impair olfactory learning without affecting the ability to recognize an olfactory cue. In vitro, two formamidines were found to inhibit octopamine-stimulated adenylate cyclase without affecting the basal activity of the enzyme, while a third one was found to stimulate adenylate cyclase; this stimulation was blocked by phentolamine and to a lesser degree by propranolol, thus resembling the effect of octopamine. The binding of [3H]octopamine toDrosophilahead membranes was also inhibited. Taken together, our results indicate that formamidines interact with octopaminergic systems inDrosophila, exert both peripheral and central effects in the fly, and could be used to dissect the roles of octopamine in development and behavior, including behavioral plasticity. The results also suggest that formamidines could be used to select mutants in aminergic transmission and in the cAMP cascade.