A human hepatitis B viral enhancer element.
A human hepatitis B viral enhancer element.
复制标题
DOI：
10.1002/j.1460-2075.1985.tb03646.x
复制
发表时间：
1985-02
期刊：
影响因子：
--
通讯作者：
Y. Shaul;W. Rutter;O. Laub
中科院分区：
文献类型：
--
作者：
Y. Shaul;W. Rutter;O. Laub
体内检测克隆的乙肝病毒基因组片段是否存在转录增强子元件。我们证明了位于HBcAg基因启动子上游约450bp的序列是其有效活性所必需的。当这些乙肝病毒刺激序列插入到异源SV40早期启动子的上游时，就会激活转录。像其他已知的增强子元件一样，该HBVDNA序列的作用方式与方向无关。此外，乙肝病毒增强子元件在人肝癌细胞系中表现出较好的活性。
Fragments of the cloned hepatitis B virus (HBV) genome were assayed in vivo for the presence of a transcriptional enhancer element. We demonstrate that sequences positioned approximately 450 bp upstream from the HBcAg gene promoter are required for its efficient activity. These HBV stimulatory sequences activate transcription when inserted upstream to a heterologous SV40 early promoter. Like other known enhancer elements, this HBV sequence acts in an orientation‐independent manner. Furthermore, the HBV enhancer element exhibits a preferred activity in a human hepatoma cell line.