Local processing in neurites of VGluT3-expressing amacrine cells differentially organizes visual information.
Local processing in neurites of VGluT3-expressing amacrine cells differentially organizes visual information.
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DOI：
10.7554/elife.31307
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发表时间：
2017-10-12
期刊：
影响因子：
7.7
通讯作者：
Kerschensteiner D
中科院分区：
文献类型：
--
作者：
Hsiang JC;Johnson KP;Madisen L;Zeng H;Kerschensteiner D
神经元在广泛的轴突上接受突触输入。信息是如何跨树枝组织的，以及神经突起中的局部处理如何对电路功能做出贡献，我们大多不得而知。在这里，我们使用双光子钙离子成像技术研究了表达VGluT3的小鼠视网膜无长突细胞(VG3-ACS)的视觉加工过程。对比度偏好(开与关)在不同的VG3-AC臂上根据神经突起的板层位置而不同，开反应喜欢较大的刺激而不是关反应。尽管相邻细胞的树枝广泛重叠，但成像群体活动显示了VG3-AC神经丛中视觉空间的连续地形图。所有VG3-AC神经突起对物体运动反应强烈，但在全局图像运动时保持沉默。因此，VG3-AC支架分别限制了对比度和位置信息的垂直和横向整合。我们认为，这种局部处理使密集的VG3-AC神经丛能够向不同的目标提供精确的物体运动信号，而不会扭曲目标特定的对比度偏好和空间接受野。
Neurons receive synaptic inputs on extensive neurite arbors. How information is organized across arbors and how local processing in neurites contributes to circuit function is mostly unknown. Here, we used two-photon Ca2+ imaging to study visual processing in VGluT3-expressing amacrine cells (VG3-ACs) in the mouse retina. Contrast preferences (ON vs. OFF) varied across VG3-AC arbors depending on the laminar position of neurites, with ON responses preferring larger stimuli than OFF responses. Although arbors of neighboring cells overlap extensively, imaging population activity revealed continuous topographic maps of visual space in the VG3-AC plexus. All VG3-AC neurites responded strongly to object motion, but remained silent during global image motion. Thus, VG3-AC arbors limit vertical and lateral integration of contrast and location information, respectively. We propose that this local processing enables the dense VG3-AC plexus to contribute precise object motion signals to diverse targets without distorting target-specific contrast preferences and spatial receptive fields.