The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors
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DOI：
10.1128/jb.185.1.243-253.2003
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发表时间：
2003-01-01
影响因子：
3.2
通讯作者：
Paddon, C
中科院分区：
文献类型：
--
作者：
Helmann, JD;Wu, MFW;Paddon, C
枯草芽孢杆菌对低水平的过氧化物表现出复杂的适应性反应。我们使用全局转录分析来监测暴露于过氧化氢 (11202) 或叔丁基过氧化物 (t-buOOH) 后 mRNA 群体变化的幅度和动力学。过氧化物刺激主要是由 PerR、sigma(B) 和 OhrR 转录因子控制的三个调节子造成的。 PerR 调节子的三个成员（katA、mrgA 和 zosA）受 H2O2 强烈诱导，受 t-buOOH 弱诱导。 PerR 调节子的其余成员仅通过过氧化物处理适度上调。总体而言，Peril 调节子基因的过氧化物诱导程度与 perR 突变株中的去抑制程度非常一致。 sigma(B) 调节子可被 58 μM H2O2 激活，但不会被 8 μM H2O2 激活，并被 t-buOOH 或（在对照实验中）叔丁醇强烈激活。除了 sigma(B) 调节子之外，还有一个基因 ohrA，它受到 t-buOOH 暴露的强烈而快速的诱导。该基因由过氧化物感应阻遏物 OhrR 控制，不受任何其他测试条件的诱导。
Bacillus subtilis exhibits a complex adaptive response to low levels of peroxides. We used global transcriptional profiling to monitor the magnitude and kinetics of changes in the mRNA population after exposure to either hydrogen peroxide (11202) or tert-butyl peroxide (t-buOOH). The peroxide stimulons could be largely accounted for by three regulons controlled by the PerR, sigma(B), and OhrR transcription factors. Three members of the PerR regulon (katA, mrgA, and zosA) were strongly induced by H2O2 and weakly induced by t-buOOH. The remaining members of the PerR regulon were only modestly up-regulated by peroxide treatment. Overall, the magnitude of peroxide induction of Peril regulon genes corresponded well with the extent of derepression in a perR mutant strain. The sigma(B) regulon was activated by 58 muM H2O2 but not by 8 muM H2O2 and was strongly activated by either t-buOOH or, in a control experiment, tert-butyl alcohol. Apart from the sigma(B) regulon there was a single gene, ohrA, that was strongly and rapidly induced by t-buOOH exposure. This gene, controlled by the peroxide-sensing repressor OhrR, was not induced by any of the other conditions tested.