Magnetic Hyperthermia Using Self-Controlled Heating Elements Consisting of Fe-Al Milling Alloy Induces Cancer Cell Apoptosis while Preserving Skeletal Muscle
Magnetic Hyperthermia Using Self-Controlled Heating Elements Consisting of Fe-Al Milling Alloy Induces Cancer Cell Apoptosis while Preserving Skeletal Muscle
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DOI：
10.1159/000501524
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发表时间：
2019-12-01
期刊：
影响因子：
5
通讯作者：
Kuniyasu, Hiroki
中科院分区：
文献类型：
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作者：
Kawahara, Isao;Goto, Kei;Kuniyasu, Hiroki
诱导坏死的抗癌药物在细胞坏死过程中促进高迁移率族蛋白1(HMGB1)的释放，而HMGB1诱导的骨骼肌自噬导致肌肉萎缩。我们评价了使用低能量磁场和自控加热元件的磁热疗(MHT)在肿瘤治疗中的疗效。通过使用热控铁铝(Fe-Al)球磨合金在43摄氏度加热小鼠皮下肿瘤，MHT诱导了细胞凋亡。相反，使用铁线的MHT在加热到大约100℃时会导致坏死。此外，使用Fe-Al球磨合金的MHT降低了茎的硬度。在使用年龄线或Fe-Al球磨合金的热疗中，两者都造成了骨骼肌的组织学退变，但观察到了质的差异。应用铁系的MHT可诱导明显的自噬，肌球蛋白重链含量降低，血清HMGB1升高。相反，使用Fe-Al球磨合金的MHT诱导了热休克蛋白90，但没有自噬，并降低了血清HMGB1。因此，应用Fe-Al球磨合金的MHT可能是一种局部治疗肿瘤以减少骨骼肌萎缩的好方法。
Necrosis-inducing anticancer drugs enhance high-mobility group box 1 (HMGB1) release during cell necrosis, and HMGB1-induced autophagy in skeletal muscle induces muscle atrophy. We evaluated the efficacy of magnetic hyperthermia therapy (MHT) using a low-energy magnetic field and self-controlled heating elements in tumor treatment. MHT-induced apoptosis by heating mouse subcutaneous tumors at 43 degrees C using a heat-controlling iron-aluminum (Fe-Al) milling alloy. In contrast, MHT using Fe line-induced necrosis by heating to approximately 100 degrees C. Furthermore, MHT with Fe-Al milling alloy reduced stemness. In hyperthermia using age line or Fe-Al milling alloy, both of them provided histological degeneration in skeletal muscle; however, qualitative differences were observed. MHT using Fe-line induced pronounced autophagy, decrease of myosin heavy chain content, and increase in serum HMGB1. In contrast, MHT using Fe-Al milling alloy induced heat shock protein 90 but no autophagy and decreased serum HMGB1. Therefore, MHT using Fe-Al milling alloy might be a good method for local treatment of tumors to reduce skeletal muscle atrophy.