Culture of C3A cells in alginate beads for fluidized bed Bioartificial liver
Culture of C3A cells in alginate beads for fluidized bed Bioartificial liver
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DOI：
10.1016/j.transproceed.2007.08.062
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发表时间：
2007-11-01
影响因子：
0.9
通讯作者：
Werynski, A.
中科院分区：
文献类型：
--
作者：
Kinasiewicz, A.;Gautier, A.;Werynski, A.
体外生物人工肝的设计目的是维持急性肝衰竭患者的肝脏解毒和合成功能，直到肝移植或自身再生。流化床生物人工肝提高了培养基和肝细胞之间的传质速度。肝脏的去铁功能可以完全由人工系统代替，但肝细胞的合成功能只能由代谢活跃的细胞获得。本研究的目的是探讨在流化床生物人工肝中，藻酸盐珠中培养的C3A细胞对合成功能的影响。使用我们自己设计的静电液滴发生器，使用低粘度藻酸盐制备藻酸盐珠中的细胞。将珠培养24小时，然后在静态条件下培养7天，然后在生物反应器中流化24小时以评估白蛋白产生。我们观察到显着增加白蛋白生产的C3A细胞包埋在藻酸盐珠在静态培养。与静态培养相比，流化培养提高了白蛋白的产量。静态培养7天后进行流化导致白蛋白合成显著增加。总之，藻酸盐珠托管肝细胞的静态培养有利于细胞功能的恢复。
Extracorporeal bioartificial liver has been designed to sustain the detoxification and synthetic function of the failed liver in patients suffering from acute liver failure until the time of liver allotransplantation or regeneration of their own. A fluidized bed, bioartificial liver improves the mass transfer velocity between the medium and the hepatocytes. Detoxification functions of the liver could be replaced by completely artificial systems, but the synthetic functions of hepatocytes may be obtained only by metabolically active cells. The aim of our study was to investigate the influence of C3A cell culture in alginate beads on synthetic function in a fluidized bed, bioartificial liver. Cells in alginate beads were prepared using an electrostatic droplet generator of our own design using low-viscosity alginate. Beads were cultured for 24 hours then 7 days in static conditions and then 24 hours of fluidization in the bioreactor to assess albumin production. We observed significantly increased albumin production by C3A cells entrapped in alginate beads during static culture. Fluidization increased albumin production compared with static culture. Fluidization performed after 7 days of static culture resulted in a significant increase in albumin synthesis. In conclusion, static culture of alginate beads hosting hepatic cells facilitates restoration of cell function.