The effect of distance on long-range chromatin interactions
The effect of distance on long-range chromatin interactions
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DOI：
10.1016/s1097-2765(00)80014-3
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发表时间：
1997-12-01
期刊：
影响因子：
16
通讯作者：
Grosveld, F
中科院分区：
文献类型：
--
作者：
Dillon, N;Trimborn, T;Grosveld, F
我们已经使用基因竞争来区分人类β-珠蛋白基因座的基因的转录激活的可能机制。第二个β-珠蛋白基因在基因座的不同位点的插入表明，更近端的β基因更有效地竞争基因座控制区（LCR）的激活。减少基因和LCR之间的相对距离降低了近端基因的竞争优势，这一结果支持LCR和基因之间直接相互作用的激活。初级转录物的可视化显示转录水平与转录周期的频率成比例，并且这种周期在体内持续约8分钟。我们还发现，β-珠蛋白基因在该位点的位置是重要的正确的发育调控。
We have used gene competition to distinguish between possible mechanisms of transcriptional activation of the genes of the human beta-globin locus. The insertion of a second beta-globin gene at different points in the locus shows that the more proximal beta gene competes more effectively for activation by the locus control region (LCR). Reducing the relative distance between the genes and the LCR reduces the competitive advantage of the proximal gene, a result that supports activation by direct interaction between the LCR and the genes. Visualization of the primary transcripts shows that the level of transcription is proportional to the frequency of transcriptional periods and that such periods last approximately 8 min in vivo. We also find that the position of the beta-globin gene in the locus is important for correct developmental regulation.