Immunoreactivity of Canine Liposarcoma to Muscle and Brown Adipose Antigens
Immunoreactivity of Canine Liposarcoma to Muscle and Brown Adipose Antigens
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DOI：
10.1177/0300985817723691
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发表时间：
2017-11-01
影响因子：
2.4
通讯作者：
Reilly, Christopher M.
中科院分区：
文献类型：
--
作者：
LaDouceur, Elise E. B.;Stevens, Sarah E.;Reilly, Christopher M.
脂肪肉瘤、横纹肌肉瘤和冬眠瘤具有一些重叠的组织学和免疫组织化学特征。尽管免疫组织化学 (IHC) 常用于诊断这些肿瘤，但除横纹肌肉瘤外，在人类脂肪肉瘤和犬冬眠瘤中也有肌肉标志物表达的报道。因此，这些肿瘤是一个诊断挑战，但由于预后和治疗的差异，区分这些肿瘤很重要。横纹肌肉瘤和脂肪肉瘤都是恶性的，但横纹肌肉瘤的转移潜力更高。相反，冬眠瘤是良性的，复发风险较低。本研究使用 IHC 调查了 25 例犬脂肪肉瘤中肌肉标记物结蛋白、肌细胞生成素和平滑肌肌动蛋白 (α-SMA) 以及棕色脂肪标记物解偶联蛋白 1 (UCP1) 的表达。进行油红O组织化学检查以确认脂质的存在，并在分化不佳的病例中诊断脂肪肉瘤。 25例中，分化良好的脂肪肉瘤15例，多形性脂肪肉瘤5例，粘液样脂肪肉瘤3例，去分化脂肪肉瘤2例。通过 IHC，25 个中的 23 个表达 UCP1，25 个中的 7 个表达 α-SMA，25 个中的 7 个表达 desmin，25 个中的 3 个表达肌细胞生成素，但抗原表达和肿瘤亚型没有明确的关系。这些发现阐明了犬脂肪肉瘤的免疫组织化学特征，并表明脂肪肉瘤、冬眠瘤和横纹肌肉瘤之间几种肌肉抗原和 UCP1 的表达存在重叠。
Liposarcoma, rhabdomyosarcoma, and hibernoma share some overlapping histologic and immunohistochemical features. Although immunohistochemistry (IHC) is commonly used in the diagnosis of these neoplasms, expression of muscle markers has been reported in human liposarcoma and canine hibernoma in addition to rhabdomyosarcoma. Thus, these neoplasms are a diagnostic challenge but important to distinguish because of differences in prognosis and treatment. Rhabdomyosarcoma and liposarcoma are both malignant, but rhabdomyosarcoma has a higher potential for metastasis. In contrast, hibernomas are benign with low risk of recurrence. This study investigated expression of the muscle markers desmin, myogenin, and -smooth muscle actin (alpha-SMA) and the brown fat marker uncoupling protein 1 (UCP1) in 25 cases of canine liposarcoma using IHC. Oil red O histochemistry was performed to confirm the presence of lipid and the diagnosis of liposarcoma in cases that were not well-differentiated. The 25 cases included 15 well-differentiated, 5 pleomorphic, 3 myxoid, and 2 dedifferentiated subtypes of liposarcoma. By IHC, 23 of 25 expressed UCP1, 7 of 25 expressed alpha-SMA, 7 of 25 expressed desmin, and 3 of 25 expressed myogenin with no clear relationship of antigen expression and tumor subtype. These findings clarify the immunohistochemical profile of canine liposarcoma and suggest overlap in the expression of several muscle antigens and UCP1 between liposarcoma, hibernoma, and rhabdomyosarcoma.