Establishment and characterization of a new embryonic cell line fromHelicoverpa armigera(Hubner)
Establishment and characterization of a new embryonic cell line fromHelicoverpa armigera(Hubner)
复制标题
棉铃虫新胚胎细胞系的建立和表征（Hubner）
DOI：
10.1007/s11626-020-00473-2
复制
发表时间：
2020
影响因子：
2.1
通讯作者：
Zhu Zhihui
中科院分区：
文献类型：
--
作者：
Zhou Lin;Li Yunfei;Wang Heyuan;Zhou Yicheng;Zhu Zhihui
Here, a new cell line, Ha168, was established fromHelicoverpa armigeraeggs and has been stably subcultured for over 30 passages in TNM-FH medium supplemented with 10% fetal bovine serum. The cell line consists of round and spindle-shaped cells and several giant cells. The round cells, with a cell diameter of 14.30 ± 2.804 μm, account for 77% of the cells. DNA amplification fingerprinting, random amplified polymorphic DNA analysis, and analysis of the mitochondrial cytochrome c oxidase subunit I gene confirmed that the Ha168 cells were derived fromH. armigera. Karyotype analysis revealed the average chromosome number of Ha168 cells to be 71. Growth curves at passage 25 were determined and demonstrated that the cell population doubling time is 56.8 h. No mycoplasma contamination was detected in the cell line. Ha168 cells can be infected by recombinant baculovirus AcMNPV-EGFP, and exogenous protein expression level in this cell line is 70% of that in the Sf9 cell line.
