Characterization of phenolic compounds in Chinese hawthorn (Crataegus pinnatifida Bge. var. major) fruit by high performance liquid chromatography-electrospray ionization mass spectrometry
Characterization of phenolic compounds in Chinese hawthorn (Crataegus pinnatifida Bge. var. major) fruit by high performance liquid chromatography-electrospray ionization mass spectrometry
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DOI：
10.1016/j.foodchem.2010.02.002
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发表时间：
2010-08-15
期刊：
影响因子：
8.8
通讯作者：
Kallio, Heikki
中科院分区：
文献类型：
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作者：
Liu, Pengzhan;Yang, Baoru;Kallio, Heikki
山楂（Crataegus spp.）果实作为营养保健品和功能性食品的原料越来越受欢迎。作为山楂的主要生物活性成分，其果实中的酚类化合物迄今为止尚未得到很好的表征。中国主要山楂品种Crataegus pinnatifida Bge的果实经80%乙醇水溶液提取后，提取酚类物质。变种通过聚酰胺柱色谱法分离主要成分，然后通过高效液相色谱法结合二极管阵列紫外光谱法和电喷雾电离质谱法进行分析。通过紫外光谱、质谱和参考化合物鉴定了 Ideain（花青素-3-O-半乳糖苷）、绿原酸、原花青素 B2 [表儿茶素-(4 beta -> 8)-表儿茶素]、表儿茶素、金丝桃苷（槲皮素-3-O-半乳糖苷）和异槲皮苷（槲皮素-3-O-葡萄糖苷）。此外，还根据紫外光谱和质谱初步鉴定出 35 种化合物。这些化合物主要是B型原花青素(PA)及其苷类，包括3个二聚体、3个三聚体、8个四聚体、4个五聚体、2个六聚体和2个PA单体苷、7个PA二聚体苷、1个PA三聚体苷、2个PA四聚体苷、1个PA五聚体苷和2个PA苷。槲皮素苷。这是对山楂果实中酚类化合物的首次系统研究，也是山楂中糖基化原花青素存在的首次报道。 (C) 2010 Elsevier Ltd. 保留所有权利。
Hawthorn (Crataegus spp.) fruits are increasingly popular as raw materials for nutraceuticals and functional foods. As a major group of bio-active components of hawthorn, the phenolic compounds of the fruits have not been well characterized so far. After extraction with 80% aqueous ethanol, the phenolics of the fruits of a major Chinese hawthorn variety, Crataegus pinnatifida Bge. var. major, were separated by polyamide column chromatography, followed by analyses by high performance liquid chromatography combined with diode array UV spectrometry and electrospray ionization mass spectrometry. Ideain (cyanidin-3-O-galactoside), chlorogenic acid, procyanidin B2 [epicatechin-(4 beta -> 8)-epicatechin], epicatechin, hyperoside (quercetin-3-O-galactoside) and isoquercitrin (quercetin-3-O-glucoside) were identified with UV spectra, mass spectra and reference compounds. In addition, 35 compounds were tentatively identified based on UV and mass spectra. These compounds were mostly B-type procyanidins (PA) and their glycosides including aglycons of 3 dimers, 3 trimers, 8 tetramers, 4 pentamers, 2 hexamers and 2 glycosides of PA monomers, 7 glycosides of PA dimers, 1 glycoside of a PA trimer, 2 glycosides of PA tetramers, 1 glycoside of a PA pentamer, and 2 glycosides of quercetin. This is the first systematic study of phenolic compounds in Chinese hawthorn fruits and the first report of the presence of glycosylated procyanidins in hawthorn. (C) 2010 Elsevier Ltd. All rights reserved.