Impedance spectroscopy of OmpF porin reconstituted into a mercury-supported lipid bilayer.
Impedance spectroscopy of OmpF porin reconstituted into a mercury-supported lipid bilayer.
复制标题
OmpF 孔蛋白重构为汞支持的脂质双层的阻抗谱。
作者：
L. Becucci;M. Moncelli;R. Guidelli
将通道形成蛋白OmpF孔蛋白掺入到由脂质双层通过硫脂质拴在汞电极上组成的仿生膜中，并通过电化学阻抗谱在KCl水溶液中进行了研究。将在0.7 V电位范围内从1 x 10（-2）至1 x 10（5）Hz记录的阻抗谱拟合至由四个RC网格组成的等效电路。所得到的电路元件对所施加的电位的依赖性进行解释的基础上，一个一般的近似方法的基础上的模型的带电的界面和动力学的钾和氯离子的易位的脂质双层，辅助OmpF孔蛋白。
The channel-forming protein OmpF porin was incorporated in a biomimetic membrane consisting of a lipid bilayer tethered to a mercury electrode via a thiolipid, and it was investigated in aqueous KCl by electrochemical impedance spectroscopy. The impedance spectra, recorded from 1 x 10(-2) to 1 x 10(5) Hz over a potential range of 0.7 V, were fitted to an equivalent circuit consisting of four RC meshes. The dependence of the resulting circuit elements upon the applied potential was interpreted on the basis of a general approximate approach based on a model of the electrified interphase and on the kinetics of the translocation of potassium and chloride ions across the lipid bilayer, assisted by the OmpF porin.