Studies on the Release of a Plasminogen Activator Inhibitor by Human Platelets
Studies on the Release of a Plasminogen Activator Inhibitor by Human Platelets
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人血小板释放纤溶酶原激活剂抑制剂的研究
DOI：
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发表时间：
1986
影响因子：
6.7
通讯作者：
F. Bachmann
中科院分区：
文献类型：
--
作者：
E. Kruithof;C. Tran;F. Bachmann
研究了血小板对血液中纤溶酶原激活物抑制剂（PA-抑制剂）活性的相对贡献。根据12名供体贫血小板血浆中PA抑制剂水平（3 ± 1 U/ml，平均值±95%置信限）与胶原蛋白、ADP或肾上腺素诱导血小板聚集后相应富血小板血浆中PA抑制剂水平（7 ± 1 U/ml）的差异，可以得出结论，血液中与血小板相关的PA抑制剂含量高于血浆。在胶原刺激的血小板中，PA抑制剂和β-血栓球蛋白（β-TG）的最大释放在15秒内达到，而在ADP刺激的血小板中，这两种因子的释放较慢。在贫血小板血浆中，PA抑制剂水平与P-TG水平之间无相关性。因此，血浆中发现的PA抑制剂不是来自于血液采集后刺激的血小板。复合物的形成率和放射性碘标记的组织型纤溶酶原激活剂（t-PA）或尿激酶（UK）的主要复合物的Mr，在血小板贫乏的血浆中，在血小板聚集后的富血小板血浆中或在洗涤的血小板的提取物中是相同的。此外，UK或t-PA与血浆PA-抑制剂或与来自血小板的PA-抑制剂的复合物结合至针对牛内皮细胞衍生的PA-抑制剂的固定化抗体。这些结果表明，血浆和血小板中的PA抑制剂非常相似或相同。
Summary The relative contribution of platelets to plasminogen activator inhibitor (PA-inhibitor) activity in blood was investigated. From the difference in PA-inhibitor levels in platelet-poor plasmas of 12 donors (3 ± 1 U/ml, mean ±95% confidence limits) and in the corresponding platelet-rich plasmas after induction of platelet aggregation by collagen, ADP or epinephrine (7 ± 1 U/ml), it may be concluded that a greater amount of PA-inhibitor in blood is associated with platelets than with plasma. In collagen-stimulated platelets maximal release of PA-inhibitor and of beta-thromboglobulin (β-TG) was attained within fifteen seconds, whereas in ADP-stimulated platelets the release of both factors was slower. In platelet-poor plasma no correlation was found between the level of PA-inhibitor and that of P-TG. Thus, the PA-inhibitor found in plasma is not derived from platelets that had been stimulated after blood collection. The rate of complex formation and the Mr of the principal complexes of radioiodinated tissue-type plasminogen activator (t-PA) or urokinase (UK), in platelet-poor plasma, in platelet-rich plasma after platelet aggregation or in an extract of washed platelets was the same. Moreover, complexes of UK or t-PA with plasmatic PA-inhibitor or with the PA-inhibitor(s) from platelets bound to immobilized antibodies against bovine endothelial cell-derived PA-inhibitor. These results show that the PA-inhibitors in plasma and in platelets are very similar or identical.
人血小板中α2-抗纤溶酶的存在和释放。
影响因子：
20.3
作者：
Plow,EF;Collen,D
通讯作者：
Collen,D