Sensitivity and specificity of human immunodeficiency virus rapid serologic assays and testing algorithms in an antenatal clinic in abidjan, Ivory Coast
Sensitivity and specificity of human immunodeficiency virus rapid serologic assays and testing algorithms in an antenatal clinic in abidjan, Ivory Coast
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DOI：
10.1128/jcm.39.5.1808-1812.2001
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发表时间：
2001-05-01
影响因子：
9.4
通讯作者：
Nkengasong, JN
中科院分区：
文献类型：
--
作者：
Koblavi-Dème, S;Maurice, C;Nkengasong, JN
为了评估科特迪瓦阿比让 HIV-1（非 B 亚型）感染者、HIV-2 感染者和 HIV-1-HIV-2 双重感染者的快速检测组合的人类免疫缺陷病毒 (HIV) 血清学检测算法，使用了总共 1,216 份已知 HIV 血清学状态的血清来评估四种快速检测的敏感性和特异性：确定 HIV-1/2、Capillus HIV-1/HIV-2、 HIV-SPOT 和 Genie II HIV-1/HIV-2，还包括从最近感染 HIV-1 B 亚型和非 B 亚型的患者获得的两个血清组。根据敏感性和特异性，对四种快速测定中的三种进行了并行（通过两个同时快速测定测试的血清样本）和串行（通过两个连续快速测定测试的血清样本）测试算法的前瞻性评估。所有检测均为 100% 敏感，特异性范围为 99.4 至 100%。在前瞻性评估中，并行和串行算法均具有100%的敏感性和特异性。我们的结果表明，快速测定具有高灵敏度和特异性，并且当用于并行或串行测试算法时，产生与基于酶联免疫吸附测定的测试策略相似的结果。在难以建立复杂实验室的地区，基于快速检测的艾滋病毒血清诊断可能是实施艾滋病毒预防和监测计划的一个有价值的替代方案。
To evaluate serologic testing algorithms for human immunodeficiency virus (HIV) based on a combination of rapid assays among persons with HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2 dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with known HIV serologic status were used to evaluate the sensitivity and specificity of four rapid assays: Determine HIV-1/2, Capillus HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2, Two serum panels obtained from patients recently infected with HIV-1 subtypes B and non-B were also included. Based on sensitivity and specificity, three of the four rapid assays were evaluated prospectively in parallel (serum samples tested by two simultaneous rapid assays) and serial (serum samples tested by two consecutive rapid assays) testing algorithms. All assays were 100% sensitive, and specificities ranged from 99.4 to 100%. In the prospective evaluation, both the parallel and serial algorithms were 100% sensitive and specific. Our results suggest that rapid assays have high sensitivity and specificity and, when used in parallel or serial testing algorithms, yield results similar to those of enzyme linked immunosorbent assay-based testing strategies. HIV serodiagnosis based on rapid assays may be a valuable alternative in implementing HIV prevention and surveillance programs in areas where sophisticated laboratories are difficult to establish.