ALA-induced photodynamic effect on viability, apoptosis and secretion of S100 protein, secreted by colon cancer cells in vitro
ALA-induced photodynamic effect on viability, apoptosis and secretion of S100 protein, secreted by colon cancer cells in vitro
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DOI：
10.1016/j.pdpdt.2016.07.009
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发表时间：
2016-09-01
影响因子：
3.3
通讯作者：
Sieron, Aleksander
中科院分区：
文献类型：
--
作者：
Kawczyk-Krupka, Aleksandra;Latos, Wojciech;Sieron, Aleksander
背景：S100蛋白是已被证实的癌症预后因素。在结直肠癌中，其分泌与疾病的临床分期相关。光动力疗法(PDT)被用作治疗这种特殊癌症的支持疗法。本研究旨在探讨亚致死剂量5-氨基乙酰丙酸(ALA)光动力疗法(ALA-PDT)对结肠癌细胞S100蛋白分泌的影响。SW480细胞系是一种含有局部恶性肿瘤的培养物。SW620细胞系具有高转移活性的特点。每一株都暴露于不同浓度的光敏剂前体-ALA，以及不同水平的光辐射。用四甲基偶氮唑盐比色法和乳酸脱氢酶比色法检测细胞存活率和细胞凋亡率。结果：ALA PDT作用后，SW480细胞株S100蛋白浓度下降27%，SW620细胞株S100蛋白浓度下降30%。与此同时，单独暴露在光下的细胞中S100蛋白的浓度并没有增加。与SW480相比，侵袭性更强的SW620细胞释放更高水平的S100蛋白。结论：ALA-PDT对持续性结肠癌细胞有良好的治疗作用。这种疗法导致两种结肠癌细胞株SW480和SW620中S100蛋白浓度降低。(C)2016爱思唯尔B.V.保留所有权利。
Background: S100 protein is a proven prognostic factor in cancers. In colorectal cancers, its secretion correlates with clinical stage of the disease. Photodynamic therapy (PDT) is used as a supporting therapy in treatment of this particular cancer. The main aim of our study was to estimate the effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in sublethal doses (ALA-PDT) on the secretion of S100 protein by colon cancer cells.Methods: Investigations were performed on two colon cancer cell lines. The SW480 cell line is a culture containing locally malignant cancer. The SW620 line is characterized by high metastatic activity. Each line was exposed to different concentrations of photosensitizer's precursor-ALA, and various level of light radiation. Afterwards, cell viability, using MTT and LDH assays and apoptosis of both lines was assessed. Then measurement of S100 protein concentration was performed using a 2-step enzyme immunoassay.Results: After application of ALA PDT the S100 protein concentration was reduced by 27% in SW480 cell line and by 30% in SW620 cell line. At the same time there has been no increase in the concentration of S100 protein in cells exposed to the light alone. It was demonstrated that the more aggressive line SW620 releases higher levels of S100 proteins in comparison with the line SW480.Conclusion: The outcome of this study presented beneficial effect of ALA-PDT on persistent colon cancer cells. This therapy leads to decrease of S100 protein concentration in both colon cancer cell lines: SW480 and SW620. (C) 2016 Elsevier B.V. All rights reserved.