C5b-9-targeted molecular MR imaging in rats with Heymann nephritis: a new approach in the evaluation of nephrotic syndrome.
C5b-9-targeted molecular MR imaging in rats with Heymann nephritis: a new approach in the evaluation of nephrotic syndrome.
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海曼肾炎大鼠 C5b-9 靶向分子 MR 成像：评估肾病综合征的新方法
DOI：
10.1371/journal.pone.0121244
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xiao W
中科院分区：
文献类型：
--
作者：
Huang Q;Wen S;Wang B;Wang Q;Guo C;Wu X;Zhang R;Yang R;Chen F;Xiao W
膜性肾病（MN）是成人肾病综合征的主要病因，严重影响患者的生活质量。目前，需要经皮肾活检来明确诊断MN。然而，这种技术是侵入性的，可能会导致严重的并发症。因此，临床上迫切需要对肾脏状态进行动态无创监测。深入的分子成像研究可以帮助找到解决方案。膜攻击复合物C5 b-9是MN发生的关键因素，该蛋白主要沉积在肾小球中。本研究采用超小超顺磁性氧化铁（USPIO）纳米颗粒结合C5 b-9的多克隆抗体，获得C5 b-9靶向磁共振分子成像探针。将探针静脉内注射到患有海曼肾炎（一种典型的MN疾病模型）的大鼠中。与非靶向组和对照组相比，注射后24小时，使用7.0特斯拉磁共振成像的体内肾脏T2* 加权成像中的信号强度显著降低。这种信号变化与病理性肾小球中纳米颗粒沉积的发现一致。这项研究证明了一种新的分子成像技术的评估MN。
Membranous nephropathy (MN) is the major cause of adult nephrotic syndrome, which severely affects patients’ quality of life. Currently, percutaneous renal biopsy is required to definitively diagnose MN. However, this technique is invasive and may cause severe complications. Therefore, an urgent clinical need exists for dynamic noninvasive monitoring of the renal state. In-depth molecular imaging studies could assist in finding a solution. Membrane attack complex C5b-9 is the key factor in the development of MN, and this protein primarily deposits in the glomerulus. The present study bound polyclonal antibodies to C5b-9 with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to obtain C5b-9-targeted magnetic resonance molecular imaging probes. The probes were injected intravenously into rats with Heymann nephritis, a classic disease model of MN. The signal intensity in the T2*-weighted imaging of kidneys in vivo using 7.0 Tesla magnetic resonance imaging decreased significantly 24 hours after injection compared to the untargeted and control groups. This signal change was consistent with the finding of nanoparticle deposits in pathological glomeruli. This study demonstrated a novel molecular imaging technique for the assessment of MN.
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