Development of novel magnetic nano-carriers for high-performance affinity purification
Development of novel magnetic nano-carriers for high-performance affinity purification
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DOI：
10.1016/j.colsurfb.2008.01.013
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发表时间：
2008-07-15
影响因子：
5.8
通讯作者：
Handa, Hiroshi
中科院分区：
文献类型：
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作者：
Nishio, Kosuke;Masaike, Yuka;Handa, Hiroshi
我们开发了直径约180mN的新型磁性纳米载体用于亲和纯化。制备的磁性纳米载体具有均匀的核/壳/壳纳米结构，由40 nm磁铁矿粒子/聚(苯乙烯-甲基丙烯酸缩水甘油酯)/聚甲基丙烯酸缩水甘油酯(GMA)通过胶束聚合制备而成。利用相对较大的40 nm磁铁矿颗粒和较大的磁化强度，磁性纳米载体可以对永磁体表现出良好的响应。磁性纳米载体具有均匀的聚合物壳层，具有很高的物理/化学稳定性，可以在不破坏核/壳结构的情况下分散在多种有机溶剂中，并能固定化各种药物。我们以抗癌药物甲氨蝶呤(MTX)为配体，制备了磁性纳米载体，并进行了亲和纯化实验。与商用磁珠相比，我们的磁性纳米载体表现出更高的纯化效率，包括非特异性结合蛋白的程度。(C)2008爱思唯尔B.V.保留所有权利。
We developed novel magnetic nano-carriers around 180 mn in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nanocarriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein. (c) 2008 Elsevier B.V. All rights reserved.