Saponin polymorphism in the Korean wild soybean (Glycine soja Sieb. and Zucc.)
Saponin polymorphism in the Korean wild soybean (Glycine soja Sieb. and Zucc.)
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DOI：
10.1111/pbr.12016
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发表时间：
2013-02
期刊：
影响因子：
2
通讯作者：
K. Panneerselvam;C. Tsukamoto;Nozomi Honda;A. Kikuchi;Jeong-Dong Lee;S. Yang;G. Chung
中科院分区：
文献类型：
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作者：
K. Panneerselvam;C. Tsukamoto;Nozomi Honda;A. Kikuchi;Jeong-Dong Lee;S. Yang;G. Chung
3025野生大豆种子皂苷成分分析。采用薄层色谱法分析了从韩国9个地区收集的植物资料，确定了其多态性变异和地理分布，并发现了皂苷成分的突变体。种子下胚轴的皂苷组成主要分为Aa、Ab、AaBc、AbBc、Aa+a、AaBc+a和AbBc+a 7种表型。主要表型为AaBc（55%）、Aa（33%）、AaBc+a（7.5%）和Aa+a（3.3%）。Ab、AbBc和AbBc+a的频率很低（0.3-0.5%）。共显性等位基因sg - 1a、sg - 1b和显性等位基因Sg-4的占比分别为98.6%、1.1%和63.3%。除突变体外，Sg-3和Sg-5等位基因在所有分析材料中均为显性。通过LC-PDA/MS/MS检测发现3个突变体。添加物CWS0115不产生皂苷Aa和Ax， CWS2133不产生皂苷Aa和Ab， CWS5095不产生任何A族皂苷。这些新发现的突变体可用于生产口感优良的大豆新品种，并可用于生物合成研究。
Seed saponin composition of 3025 wild soybean (Glycine soja Sieb. and Zucc.) accessions collected from nine regions of Korea was analysed by thin-layer chromatography to determine its polymorphic variation and geographical distribution and find mutants in saponin components. The saponin composition of seed hypocotyls was primarily divided into seven phenotypes, designated as Aa, Ab, AaBc, AbBc, Aa+a, AaBc+a and AbBc+a. The predominant phenotypes were AaBc (55%), Aa (33%), AaBc+a (7.5%) and Aa+a (3.3%). The frequencies of Ab, AbBc and AbBc+a were very low (0.3-0.5%). Codominant alleles Sg-1 a and Sg-1 b and dominant allele Sg-4 occupied 98.6, 1.1 and 63.3%, respectively. Alleles Sg-3 and Sg-5 were found to be dominant in all the analysed accessions except the mutants. Three accessions were discovered as mutants via LC-PDA/MS/MS. The accession CWS0115 did not produce saponin Aa and Ax, CWS2133 did not produce saponin Aa and Ab and CWS5095 did not produce any group A saponins. These newly determined mutants might be utilized in producing a new soybean variety with good taste as well as in biosynthetic studies.