Detection of apoptosis in fusing versus nonfusing mouse cranial sutures.
Detection of apoptosis in fusing versus nonfusing mouse cranial sutures.
复制标题
融合与非融合小鼠颅缝中细胞凋亡的检测。
DOI：
10.1097/01.scs.0000168759.61947.7f
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Gosain,ArunK
中科院分区：
文献类型：
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作者：
Agresti,Michael;Gosain,ArunK
细胞凋亡可能参与维持缝合通畅。在小鼠中，后额叶缝线在出生后45天融合，而所有剩余的颅骨缝线保持通畅。无论是在体内还是在体外，没有发表的报告表明，在出生后第6天之后，融合和未融合的小鼠颅骨缝合线在细胞凋亡方面存在差异。在目前的研究中，我们假设细胞凋亡是维持缝合通畅所必需的。我们预测在小鼠正常缝合融合后，后额叶缝合处的凋亡细胞应少于矢状面缝合处。我们还假设所有缝合线在缝合线融合前凋亡细胞的数量和排列应该是相似的。在出生后第25和45天研究后额、矢状面缝合。通过DNA片段化或末端脱氧核苷酸转移酶介导的dUTP镍端标记实验，以及特异性凋亡蛋白BCL-10的存在，将出生后45天的小鼠定位在矢状缝线颅骨边缘。这些凋亡标记在出生后45天小鼠的融合后额叶缝线中未见。出生第25天小鼠后额、矢状缝间质凋亡细胞数量相近。这些数据表明，在小鼠后额叶缝合融合期后，未闭合的矢状缝中存在凋亡细胞。我们得出结论，细胞凋亡是维持小鼠颅骨缝合线通畅的重要组成部分。
Apoptosis may be involved in maintenance of suture patency. In mice, the posterior frontal suture fuses by postnatal day 45, whereas all remaining cranial sutures remain patent. There are no published reports documenting differences in apoptosis between fusing and nonfusing mouse cranial sutures beyond postnatal day 6 either in vivo or in vitro. In the current study, we hypothesized that apoptosis is required for maintenance of suture patency. We predicted that after normal suture fusion in the mouse, the posterior frontal suture should have fewer apoptotic cells than the sagittal suture. We also hypothesized that all of the sutures should look similar with respect to the number and arrangement of apoptotic cells before suture fusion. The posterior frontal and sagittal sutures were studied on postnatal days 25 and 45. The fragmentation of DNA or terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay assay, as well as the presence of BCL-10, a specific apoptotic protein, were localized to the leading edge of the sagittal suture calvaria of postnatal day 45 mice. These apoptotic markers were not visualized within the fused posterior frontal suture of postnatal day 45 mice. Posterior frontal or sagittal suture mesenchyme of postnatal day 25 mice showed similar amounts of apoptotic cells. These data indicate that apoptotic cells are present in the patent sagittal suture beyond the period of posterior frontal suture fusion in the mouse. We conclude that apoptosis is an integral component to maintain suture patency in the mouse calvaria.