Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors
Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors
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DOI：
10.1128/aem.00402-06
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发表时间：
2006-08-01
影响因子：
4.4
通讯作者：
Francis, Christopher A.
中科院分区：
文献类型：
--
作者：
Park, Hee-Deung;Wells, George F.;Francis, Christopher A.
我们报告的分子证据表明，氨氧化古菌（AOA）发生在活性污泥生物反应器用于去除废水中的氨。使用针对古细菌氨单加氧酶亚基A（amoA）基因的PCR引物，我们检索和比较了75个序列从5个污水处理厂低溶解氧水平和长停留时间运行。这些序列与土壤和沉积物中的序列相似，主要分布在4个系统发育簇中。其中一个簇包含从所有五个活性污泥样品（来自俄勒冈州、威斯康星州、宾夕法尼亚州和新泽西）获得的几乎相同的amoA序列，占所有序列的67%，表明这种AOA酶类型可能广泛存在于硝化生物反应器中。
We report molecular evidence that ammonia-oxidizing archaea (AOA) occur in activated sludge bioreactors used to remove ammonia from wastewater. Using PCR primers targeting archaeal ammonia monooxygenase subunit A (amoA) genes, we retrieved and compared 75 sequences from five wastewater treatment plants operating with low dissolved oxygen levels and long retention times. All of these sequences showed similarity to sequences previously found in soil and sediments, and they were distributed primarily in four major phylogenetic clusters. One of these clusters contained virtually identical amoA sequences obtained from all five activated sludge samples (from Oregon, Wisconsin, Pennsylvania, and New Jersey) and accounted for 67% of all the sequences, suggesting that this AOA phylotype may be widespread in nitrifying bioreactors.