Measurement of stress and strain applied to electrochemically aligned collagen fibers
Measurement of stress and strain applied to electrochemically aligned collagen fibers
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测量施加于电化学排列的胶原纤维的应力和应变
DOI：
10.1117/12.894151
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
S.Inoue
中科院分区：
文献类型：
--
作者：
K.Yoshiki;N.Goami;T.Namazu;S.Inoue
在这项研究中，我们开发了一种新的应变测量技术，用于电化学排列胶原蛋白（ELAC）纤维，使用二次谐波生成（SHG）显微镜。采用典型的电化学方法制备了ELAC纤维，并进行了交联。为了与天然胶原纤维进行比较，我们评估了制备的ELAC纤维和人类跟腱的极化依赖性。结果表明，由于交联，ELAC纤维表现出类似于肌腱的极化依赖性，但仅在靠近肌腱的区域。SHG与外加应变之间的关系是通过SHG显微镜和拉伸试验相结合来确定的。由于外加应力的作用，ELAC纤维的SHG在高应变区发生了变化。
In this study, we developed a novel strain measurement technique for electrochemically aligned collagen (ELAC) fibres using second harmonic generation (SHG) microscopy. The ELAC fibres were prepared by a typical electrochemical method and were subjected to cross-linking. For comparison with natural collagen fibres, polarization dependency of the prepared ELAC fibres and that of a human Achilles' tendon were evaluated. The results showed that, because of crosslinking, the ELAC fibres exhibit polarization dependency similar to that of the tendon but only in a region close to the tendon. The relationship between SHG and the applied strain was determined by a combination of SHG microscopy and tensile tests. The SHG from the ELAC fibres changed in the high strain region because of the applied stress.