Colonic mucin release in response to immobilization stress is mast cell dependent
Colonic mucin release in response to immobilization stress is mast cell dependent
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DOI：
10.1152/ajpgi.1998.274.6.g1094
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发表时间：
1998-06-01
影响因子：
4.5
通讯作者：
Pothoulakis, C
中科院分区：
文献类型：
--
作者：
Castagliuolo, I;Wershil, BK;Pothoulakis, C
我们最近报道了制动应激增加了大鼠结肠的结肠运动性、粘蛋白和前列腺素E-2（PGE（2））释放以及粘膜肥大细胞脱粒[Proc.Natl.2004]。Acad. Sci. USA 93：12611-12615，1996; Am. J. Physiol.271（Gastrointest. 34）：G884-G892，1996]。为了直接评估肥大细胞的贡献，我们比较了肥大细胞缺陷Kit（W）/Kit（W-v）和正常（+/+）小鼠对应激的结肠反应。在固定应激30分钟后，测量了Kit（W）/Kit（W-v）和（+/+）小鼠培养的结肠外植体中粘蛋白和PGE（2）的释放。我们发现，应激刺激结肠粘蛋白释放（1.8倍），杯状细胞消耗（3倍）和PGE（2）（2.3倍）释放（+/+），但不是肥大细胞缺陷的Kit（W）/Kit（W-v）小鼠。然而，通过注射来自（+/+）小鼠的骨髓来源的肥大细胞重建肥大细胞群的肥大细胞缺陷小鼠对应激的结肠反应与正常（+/+）小鼠相似。相比之下，通过粪便排出量估计的响应于应激的结肠运输变化在Kit（W）/Kit（W-v）和正常（+/+）小鼠之间是相似的。我们的结论是，肥大细胞调节结肠粘蛋白和PGE（2）的释放，但不调节结肠运输对免疫应激的反应。
We recently reported that immobilization stress increased colonic motility, mucin, and prostaglandin E-2 (PGE(2)) release and mucosal mast cell degranulation in rat colon [Proc. Natl. Acad. Sci. USA 93: 12611-12615, 1996; Am. J. Physiol. 271 (Gastrointest. Liver Physiol. 34): G884-G892, 1996]. To directly assess the contribution of mast cells, we compared colonic responses to stress in mast cell-deficient Kit(W)/Kit(W-v) and normal (+/+) mice. Mucin and PGE(2) release were measured in colonic explants cultured from Kit(W)/Kit(W-v) and (+/+) mice 30 min after immobilization stress. We found that stress stimulated colonic mucin release (1.8-fold), goblet cell depletion (3-fold), and PGE(2) (2.3-fold) release in (+/+) but not mast cell-deficient Kit(W)/Kit(W-v) mice. However, mast cell-deficient mice that had their mast cell population reconstituted by injection of bone marrow-derived mast cells from (+/+) mice had colonic responses to stress similar to those of normal (+/+) mice. In contrast, colonic transit changes in response to stress, estimated by fecal output, were similar between Kit(W)/Kit(W-v) and normal (+/+) mice. We conclude that mast cells regulate colonic mucin and PGE(2) release but not colonic transit changes in responsetom immunobilization stress.