Sensing Actin Dynamics through Adherens Junctions
Sensing Actin Dynamics through Adherens Junctions
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DOI：
10.1016/j.celrep.2020.01.106
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发表时间：
2020-02-25
期刊：
影响因子：
8.8
通讯作者：
Troyanovsky, Sergey M.
中科院分区：
文献类型：
--
作者：
Indra, Indrajyoti;Troyanovsky, Regina B.;Troyanovsky, Sergey M.
我们研究了点状黏附连接(PAJ)，以确定短暂的钙粘附素簇和相对稳定的肌动蛋白束是如何相互作用的，尽管动力学上存在差异。我们发现PAJ连接的束由两个不同的区域组成--束柄(AJ-BS)和位于钙粘蛋白簇和茎之间的尖端(AJ-BT)。顶端与茎有许多不同之处：它没有肌动蛋白捆绑蛋白Calponin，并且表现出更快的F-肌动蛋白周转速度。茎中的F-肌动蛋白呈向心运动，而顶端的F-肌动蛋白则静止不动。F-肌动蛋白在顶端和茎中的周转依赖于钙粘附素簇的稳定性，而钙粘附素簇又受F-肌动蛋白的调节。钙粘附素的稳定性和相关的F-肌动蛋白之间的紧密双向耦合表明，pAJ，也许还有其他AJ，如何允许细胞感知和协调相邻细胞中肌动蛋白细胞骨架的动态--我们将这种机制称为“王朝感知”。
We study punctate adherens junctions (pAJs) to determine how short-lived cadherin clusters and relatively stable actin bundles interact despite differences in dynamics. We show that pAJ-linked bundles consist of two distinct regions-the bundle stalk (AJ-BS) and a tip (AJ-BT) positioned between cadherin clusters and the stalk. The tip differs from the stalk in a number of ways: it is devoid of the actin-bundling protein calponin, and exhibits a much faster F-actin turnover rate. While F-actin in the stalk displays centripetal movement, the F-actin in the tip is immobile. The F-actin turnover in both the tip and stalk is dependent on cadherin cluster stability, which in turn is regulated by F-actin. The close bidirectional coupling between the stability of cadherin and associated F-actin shows how pAJs, and perhaps other AJs, allow cells to sense and coordinate the dynamics of the actin cytoskeleton in neighboring cells-a mechanism we term "dynasensing.''