LOCALIZATION OF THE HEAT SHOCK-INDUCED PROTEINS IN DROSOPHILA-MELANOGASTER TISSUE-CULTURE CELLS
LOCALIZATION OF THE HEAT SHOCK-INDUCED PROTEINS IN DROSOPHILA-MELANOGASTER TISSUE-CULTURE CELLS
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DOI：
10.1016/0012-1606(80)90320-6
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
TISSIERES, A
中科院分区：
文献类型：
--
作者：
ARRIGO, AP;FAKAN, S;TISSIERES, A
D的曝光。将黑腹组织培养细胞培养至37 ° C。C诱导合成一组新的蛋白质，即热休克蛋白，而在热休克前活跃的大多数蛋白质的合成受到强烈抑制。热休克蛋白的细胞定位在不同的细胞组分和EM放射自显影的细胞切片进行了研究。22、23、26和27 kdalton的热休克蛋白和至少一部分68和70 kdalton蛋白在热休克后存在于细胞核中，它们存在于染色质和核仁制备物中。当温度变回25 ℃时，在25 ℃时，热休克蛋白质的合成在几小时后衰退并停止，而正常情况下蛋白质在25 ℃时合成。C重新出现在25度。大部分热休克蛋白离开细胞核，主要定位在细胞质中。
Exposure of D. melanogaster tissue culture cells to 37.degree. C induces synthesis of a new set of proteins, the heat shock proteins, while synthesis of most of the proteins actively made before the heat shock is strongly repressed. Cellular localization of the heat shock proteins was studied in different cell fractions and by EM autoradiography of cell sections. Heat shock proteins of 22, 23, 26 and 27 kdaltons and at least a fraction of the 68- and 70-kdalton proteins are in the nucleus after the heat shock, where they are present in chromatin and nucleoli preparations. When the temperature is shifted back to 25.degree. C, synthesis of the heat shock proteins regresses and stops after several hours, while the proteins normally synthesized at 25.degree. C reappear. At 25.degree. C a large proportion of the heat shock proteins leaves the nucleus and is predominantly localized in the cytoplasm.