The natural compound, formononetin, extracted from Astragalus membranaceus increases adipocyte thermogenesis by modulating PPARγ activity
The natural compound, formononetin, extracted from Astragalus membranaceus increases adipocyte thermogenesis by modulating PPARγ activity
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黄芪中提取的天然化合物芒柄花素通过调节 PPARgamma 活性增加脂肪细胞生热作用
DOI：
10.1111/bph.14139
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发表时间：
2018-05-01
影响因子：
7.3
通讯作者：
Wu, Donghai
中科院分区：
文献类型：
--
作者：
Nie, Tao;Zhao, Shiting;Wu, Donghai
通过脂肪细胞热发生的背景和目的的能量消耗通常被视为减轻肥胖及其相关疾病的有前途的策略。但是，很少有临床有效和安全的药物促进脂肪细胞的热发生。在这项研究中，筛选了20种传统的中草药，以检查它们是否诱导脂肪细胞的热发生。实验方法通过通过QPCR评估UNCOPLING蛋白1（UCP1）的表达，分析了从膜曲霉提取物提取物中分离出的中药art药或成分对脂肪细胞热发生的影响。将八周龄的C57BL6/J雄性小鼠喂给高脂饮食8周，然后将其随机分为两组用媒介物或Formononetin治疗的两组，持续8周。进行了葡萄糖耐受性测试和用甲氧精和曙红对脂肪组织的染色。用开开路间接量热法测量全身氧的消耗。在小鼠脂肪细胞的原发性培养物中，A.膜膜的表达增加了UCP1的表达。 Formononetin是曲霉提取物的唯一已知成分，用于增加脂肪细胞UCP1表达。与未经治疗的小鼠相比，用formononetin治疗的饮食诱导的肥胖小鼠的体重降低，并且能量消耗更高。此外，formononetin与PPAR伽马座直接结合。结论和含义结合在一起，我们的研究表明，来自A.膜的中草药及其成分的formononetin具有降低肥胖症和相关代谢性疾病的潜力。我们的结果表明，formononetin调节脂肪细胞的热发生为非经典的PPAR伽玛激动剂。
BACKGROUND AND PURPOSEIncreasing energy expenditure through adipocyte thermogenesis is generally accepted as a promising strategy to mitigate obesity and its related diseases. However, few clinically effective and safe agents are known to promote adipocyte thermogenesis. In this study, 20 traditional Chinese herbal medicines were screened to examine whether they induced adipocyte thermogenesis.EXPERIMENTAL APPROACHThe effects of Chinese herbal medicines or components isolated from extracts of A. membranaceus, on adipocyte thermogenesis were analysed by assessing expression of uncoupling protein 1 (UCP1) by qPCR. Eight-week-old C57BL6/J male mice were fed a high-fat diet for 8 weeks and then randomized to two groups treated with vehicle or formononetin for another 8 weeks. Glucose tolerance tests and staining of adipose tissue with haematoxylin and eosin were carried out. Whole-body oxygen consumption was measured with an open-circuit indirect calorimetry system.KEY RESULTSExtracts of A. membranaceus increased expression of Ucp1 in primary cultures of mouse adipocytes. Formononetin was the only known component of A. membranaceus extracts to increase adipocyte Ucp1 expression. Diet-induced obese mice treated with formononetin gained less weight and showed higher energy expenditure than untreated mice. In addition, formononetin binds directly with PPAR gamma.CONCLUSIONS AND IMPLICATIONTaken together, our study demonstrates that the Chinese herbal medicine from A. membranaceus and its constituent formononetin have the potential to reduce obesity and associated metabolic disorders. Our results suggest that formononetin regulates adipocyte thermogenesis as a non-classical PPAR gamma agonist.