The prolactin gene, but not the growth hormone gene, is located on chromosome 9 in the rat.
The prolactin gene, but not the growth hormone gene, is located on chromosome 9 in the rat.
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催乳素基因（而非生长激素基因）位于大鼠的第 9 号染色体上。
DOI：
10.1089/dna.1984.3.139
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Bancroft,FC
中科院分区：
文献类型：
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作者：
Gerhard,DS;Szabo,P;Kawasaki,E;Bancroft,FC
许多实验室采用大鼠垂体瘤细胞（GH细胞）的克隆系进行的实验表明，催乳素和生长激素的mRNA的细胞水平受到多种激素和其他因素的调节。为了进一步研究这两个基因的表达调控提供一个框架，我们研究了它们在大鼠染色体上的位置，采用我们最近开发的技术，通过与125 I标记的cDNA质粒原位直接杂交来鉴定一个独特的基因。大鼠催乳素cDNA质粒（pPRL-1）产生的信号显着高于预期的随机基础上从大鼠FRE细胞的染色体9和15。两条染色体均在9号染色体端粒附近和15号染色体短臂上显示出亚染色体簇的银颗粒。用质粒pBR 322进行的对照实验也产生了聚集在染色体15短臂上的显著信号。因此，我们得出结论，15号染色体含有序列同源的pBR 322和催乳素基因位于附近的端粒的9号染色体在大鼠。大鼠生长激素cDNA质粒（pBR 322-GH 1）产生的信号高于预期的随机基础上超过FRE细胞染色体7，13和15，虽然标记是较差的比催乳素。15号染色体上的信号可能是由于与质粒pBR 322同源的序列。在7号和13号染色体上的信号显示了在7号染色体上的着丝粒附近和13号染色体长臂上的端粒附近的清楚的亚染色体聚类。因此，我们得出结论，生长激素基因在大鼠位于一个或两个这些位置。因此，生长激素基因与大鼠的催乳素基因无关。
Experiments from a number of laboratories, employing clonal lines of rat pituitary tumor cells (GH cells), have shown that the cellular levels of the mRNAs for prolactin and growth hormone are subject to regulation by a variety of hormones and other factors. To provide a framework for further studies of the regulation of the expression of these two genes, we have studied their chromosomal locations in the rat, employing our recently developed technique for identifying a unique gene by direct hybridizationin situwith a,125I-labeled cDNA plasmid. A rat prolactin cDNA plasmid (pPRL-1) yielded a signal significantly above that expected on a random basis over chromosomes 9 and 15 from rat FRE cells. Both chromosomes exhibited a subchromosomal clustering of silver grains near the telomere of chromosome 9 and on the short arm of chromosome 15. Control experiments with plasmid pBR322 also yielded a significant signal clustered over the short arm of chromosome 15. Hence, we conclude that chromosome 15 contains sequences homologous to pBR322 and that the prolactin gene is located near the telomere of chromosome 9 in the rat. A rat growth hormome cDNA plasmid (pBR322-GH1) yielded a signal above that expected on a random basis over FRE cell chromosomes 7, 13, and 15, although the labeling was poorer than with prolactin. The signal over chromosome 15 is probably due to sequences homologous to plasmid pBR322. The signals over chromosomes 7 and 13 showed a clear subchromosomal clustering near the centromere on chromosome 7 and near the telomere on the long arm of chromosome 13. Hence, we conclude that the growth hormone gene in the rat is located at one or both of these positions. The growth hormone gene is therefore not linked to the prolactin gene in the rat.