Serial cine-magnetic resonance imaging of left ventricular remodeling after myocardial infarction in rats
Serial cine-magnetic resonance imaging of left ventricular remodeling after myocardial infarction in rats
复制标题
DOI：
10.1002/jmri.1218
复制
发表时间：
2001-11-01
影响因子：
4.4
通讯作者：
Bauer, WR
中科院分区：
文献类型：
--
作者：
Nahrendorf, M;Wiesmann, F;Bauer, WR
本研究利用磁共振成像（MRI）对完整大鼠左冠状动脉结扎后的形态学和功能变化进行了系列研究。在心肌梗死（MI）后4、8、12和16周，在7特斯拉磁铁中使用超声心动图（ECG）触发的快速低角度拍摄（FLASH）序列进行MM研究。11只Wistar大鼠与4只假手术大鼠的mi大小、左心室（LV）质量和容积、心脏指数、射血分数（EF）、远端壁和疤痕厚度进行比较。16周时进行多巴酚丁胺应激MRI （10 μ l/kg x分钟）。心肌梗死组（小心肌梗死< 30%,N = 5，大心肌梗死30%，N = 6）左室质量（小心肌梗死+ 47.8%，大心肌梗死+ 74.1%）和壁厚（大心肌梗死1.21 +/- 0.03 ~ 1.84 +/- 0.07 mm）均显著增加。疤痕厚度从4周到16周下降（大MI为0.92 +/- 0.06至0.38 +/- 0.02mm, P < 0.05）。心肌梗死组和心肌梗死组的舒张末期容积均显著升高，但只有心肌梗死大的动物的舒张末期容积在4 ~ 16周进一步升高（657.1 +/- 38.6 ~ 869.7 +/- 60.7 muL, P < 0.05）。与假手术组相比，心肌梗死组EF明显下降（心肌梗死大31.5±2.0%）。心肌梗死后4 ~ 16周，壁厚下降（大心肌梗死50.9 +/- 9.9 ~ 28.9 +/- 4.4%,P < 0.05）。应激时，假手术大鼠和心肌梗死大鼠的壁厚分别从66.5 +/- 8.2增加到111.2 +/- 6.7%，从30.8 +/- 4.3增加到47.5 +/- 5.8% （P < 0.05）。在整个观察期间，所有心肌梗死动物均出现心肌肥大，而仅在心肌梗死大的动物中，4周后出现心肌扩张。这些形态学变化伴随着EF的早期下降；以心肌壁增厚为特征的心肌功能逐渐恶化。(C) 2001 Wiley-Liss, Inc。
The purpose of the present study was the serial investigation of morphological and functional changes after left coronary artery ligation in the intact rat using cine-magnetic resonance imaging (MRI). MM studies were performed 4, 8, 12, and 16 weeks after myocardial infarction (MI) with an echocardiogram (ECG)-triggered cine-fast low-angle shot (FLASH)-sequence in a 7-Tesla magnet. MI-size, left ventricular (LV) mass and volumes, cardiac index, ejection fraction (EF), and remote wall and scar thickness of 11 Wistar rats were compared to four sham-operated rats. Stress MRI with dobutamine (10 mul/kg x minute) was performed at 16 weeks. In MI groups (small MI < 30%, N = 5, large MI > 30%, N = 6), there was significant increase of LV mass (small MI + 47.8% increase, large MI + 74.1%) and wall thickness (large MI 1.21 +/- 0.03 to 1.84 +/- 0.07 mm). Scar thickness declined from four to 16 weeks (large MI 0.92 +/- 0.06 to 0.38 +/- 0.02mm, P < 0.05). End-diastolic volume of both MI groups was significantly elevated but increased further only in animals with large MI from four to 16 weeks (657.1 +/- 38.6 to 869.7 +/- 60.7 muL, P < 0.05). Compared to sham, EF was significantly depressed in MI (large MI 31.5 +/- 2.0%). Wall thickening declined from four to 16 weeks post-MI (large MI 50.9 +/- 9.9 to 28.9 +/- 4.4%, P < 0.05). During stress, sham and MI rats increased wall thickening from 66.5 +/- 8.2 to 111.2 +/- 6.7% and from 30.8 +/- 4.3 to 47.5 +/- 5.8%, respectively (P < 0.05). Hypertrophy was found in all animals with MI throughout the entire period of observation, whereas dilatation after four weeks was only detected in animals with large MI. These morphologic changes were accompanied by an early decline of EF; myocardial function characterized by wall thickening deteriorated later. (C) 2001 Wiley-Liss, Inc.