Regulation of floral patterning and organ identity by Arabidopsis ERECTA-family receptor kinase genes
Regulation of floral patterning and organ identity by Arabidopsis ERECTA-family receptor kinase genes
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DOI：
10.1093/jxb/ert270
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发表时间：
2013-12-01
影响因子：
6.9
通讯作者：
Torii, Keiko U.
中科院分区：
文献类型：
--
作者：
Bemis, Shannon M.;Lee, Jin Suk;Torii, Keiko U.
由于缺乏细胞迁移，植物器官发生依赖于协调的细胞增殖、细胞生长和分化。花具有复杂的结构，由萼片和花瓣构成花被，雄蕊和雌蕊是雌雄配子体分化的地方。虽然我们对控制花发育的基因调控网络的理解取得了进展，但对细胞间协调如何影响花器官特化的了解相对较少。拟南芥ERECTA（ER）家族受体激酶，ER，ER-LIKE 1（ERL 1）和ERL 2，调节花序结构，器官形状和表皮气孔模式。ER家族基因共同调控花分生组织的形成和花器官的形成。干细胞标记CLAVATA 3在er erl 1 erl 2突变体的花分生组织中表现出错位表达。引人注目的是，在er erl 1 erl 2花中观察到萼片向心皮的同源异型转化。一致的是，异位表达的AGAMOUS，决定心皮的身份，检测到在erl 1 erl 2花原基。在已知的ER家族受体激酶的下游组分中，气孔图案形成也需要YODA（YDA）。YDA和ER-家族表现出复杂的、协同的遗传相互作用：ER-ERL 1-ERL 2-YDA四重突变体植物变得非常小，愈伤组织样群众。虽然组成型活性YDA完全拯救气孔聚集在er erl 1 erl 2，它只能部分拯救er erl 1 erl 2花缺陷。这项研究表明，ER家族的信号是至关重要的，以确保正确的表达域的花分生组织和花器官的身份决定因素，并进一步意味着存在一个非经典的下游途径。
Due to the lack of cell migration, plant organogenesis relies on coordinated cell proliferation, cell growth, and differentiation. A flower possesses a complex structure, with sepals and petals constituting the perianth, and stamens and pistils where male and female gametophytes differentiate. While advances have been made in our understanding of gene regulatory networks controlling flower development, relatively little is known of how cell-cell coordination influences floral organ specification. The Arabidopsis ERECTA (ER)-family receptor kinases, ER, ER-LIKE1 (ERL1), and ERL2, regulate inflorescence architecture, organ shape, and epidermal stomatal patterning. Here it is reported that ER-family genes together regulate floral meristem organization and floral organ identity. The stem cell marker CLAVATA3 exhibits misplaced expression in the floral meristems of the er erl1 erl2 mutant. Strikingly, homeotic conversion of sepals to carpels was observed in er erl1 erl2 flowers. Consistently, ectopic expression of AGAMOUS, which determines carpel identity, was detected in er erl1 erl2 flower primordia. Among the known downstream components of ER-family receptor kinases in stomatal patterning, YODA (YDA) is also required for proper floral patterning. YDA and the ER-family show complex, synergistic genetic interactions: er erl1 erl2 yda quadruple mutant plants become extremely small, callus-like masses. While a constitutively active YDA fully rescues stomatal clustering in er erl1 erl2, it only partially rescues er erl1 erl2 flower defects. The study suggests that ER-family signalling is crucial for ensuring proper expression domains of floral meristem and floral organ identity determinants, and further implies the existence of a non-canonical downstream pathway.