Imprinting and expression of Dio3os mirrors Dio3 in rat.
Imprinting and expression of Dio3os mirrors Dio3 in rat.
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DOI：
10.3389/fgene.2012.00279
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发表时间：
2012
影响因子：
3.7
通讯作者：
Herzing LB
中科院分区：
文献类型：
--
作者：
Dietz WH;Masterson K;Sittig LJ;Redei EE;Herzing LB
基因组印记是印记基因的母本或父本等位基因的优先表达，通常通过印记长非编码（lnc）“反义”RNA的表达来维持。这些可能会重叠印迹转录，并从相反的等位基因表达。以前，我们已经描述了大脑区域特异性印迹表达的Dio 3基因在大鼠，这是优先修改胎儿乙醇暴露。Dio 3 os（相对链）转录物在小鼠和人类中以与Dio 3相反的方向转录，部分重叠Dio 3启动子，并反映总Dio 3发育表达水平。在这里，我们提出，大鼠Dio 3 os转录本（S）表现出大脑区域特异性印迹表达模式类似于Dio 3。大鼠Dio 3 os转录表达也类似地通过胎儿乙醇暴露而改变。独特的是，Dio 3和Dio 3 os表达都发生在相同的等位基因上，而不是相反的等位基因上，如通过链特异性RT-PCR所确定的。未来的研究将需要直接操作的Dio 3 os转录本，以确定是否新的平行的总和等位基因特异性表达模式的正义/反义印迹基因对反映了一个尚未确定的调控机制lncRNA介导的组织特异性印迹表达，或者更确切地说是一个更直接的，但以前未描述的转录共调控过程的结果。
Genomic imprinting, the preferential expression of maternal or paternal alleles of imprinted genes, is often maintained through expression of imprinted long non-coding (lnc) “antisense” RNAs. These may overlap imprinted transcripts, and are expressed from the opposite allele. Previously we have described brain region-specific imprinted expression of the Dio3 gene in rat, which is preferentially modified by fetal ethanol exposure. The Dio3os (opposite strand) transcript is transcribed in opposite orientation to Dio3 in mouse and human, partially overlaps the Dio3 promoter, and mirrors total Dio3 developmental expression levels. Here, we present that the rat Dio3os transcript(s) exhibits brain region-specific imprinted expression patterns similar to those of Dio3. Rat Dio3os transcript expression is also similarly modified by fetal ethanol exposure. Uniquely, both Dio3 and Dio3os expression occur on the same, rather than opposite, alleles, as determined by strand-specific RT-PCR. Future studies will require direct manipulation of the Dio3os transcript to determine whether the novel paralleling of total and allele-specific expression patterns of this sense/antisense imprinted gene pair reflects an as-yet undefined regulatory mechanism for lncRNA mediated tissue-specific imprinted expression, or rather is a consequence of a more straightforward, but previously undescribed transcriptional coregulation process.