Formation of exudate droplets by Metarhizium anisopliae and the presence of destruxins
Formation of exudate droplets by Metarhizium anisopliae and the presence of destruxins
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DOI：
10.3852/09-079
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发表时间：
2010-01-01
期刊：
影响因子：
2.8
通讯作者：
Burgstaller, Wolfgang
中科院分区：
文献类型：
--
作者：
Hutwimmer, Stefan;Wang, Hui;Burgstaller, Wolfgang
金龟子绿僵菌液滴分泌的营养条件。对绿僵菌进行了研究。液滴中的渗出仅在具有一种以上碳源的培养基上发生，并且高度依赖于代谢良好的糖（如海藻糖）和非优选糖（特别是阿拉伯糖）的比率。渗出液滴含有浓度与在具有等效物的Czapek Dox培养基上的浸没培养物中的浓度相似的破坏毒素A、B和E。C：N比，但明显低于以前报道的标准Czapek Dox或Sabouraud葡萄糖肉汤。在菌丝体正下方和菌落前殖民地边缘2 cm处的琼脂样品中也发现了破坏菌素。从不同的媒体检索的渗出物被证明有Prl蛋白酶相关的酶活性。另外的HPLC分析表明，来自不同培养基的液滴在糖和酸含量上没有差异。提出了一个假设，关于绿僵菌在生长过程中在这些条件下滴液的触发。
Nutritional conditions causing droplet exudation by Metarhizium anisopliae var. anisopliae were Studied. Exudation in droplets Occurred only on media with more than one carbon Source and was highly dependent Oil the ratio of a well metabolized sugar such as trehalose and a nonpreferred sugar, in particular arabinose. Exuded droplets contained destruxin A, B and E in concentrations similar to those on submerged Culture on Czapek Dox medium with equivalent. C:N ratios but was clearly less than previously reported on standard Czapek Dox or Sabouraud dextrose broth. Destruxins also were Found in agar samples from directly below mycelium and from up to 2 cm front the colony edge. Exudates retrieved from different media were proven to have Prl protease-related enzyme activity. Additional HPLC analysis indicated that droplets from diverse media did not differ in their sugar and acid content. A hypothesis is presented regarding the trigger for guttation in Metarhizium during growth Under these conditions.