Temperature-sensitive influenza A virus clones originated by a cross between A/Aichi/2/68 (H3N2) and B/Yamagata/1/73
Temperature-sensitive influenza A virus clones originated by a cross between A/Aichi/2/68 (H3N2) and B/Yamagata/1/73
复制标题
温度敏感的甲型流感病毒克隆源自 A/Aichi/2/68 (H3N2) 和 B/Yamagata/1/73 之间的杂交
作者：
K. Tobita;T. Tanaka;H. Goto;S. Feng
摘要在流感病毒 B/Yamagata/1/73 和克隆 6-10 之间进行了遗传杂交，克隆 6-10 是一种 A 型流感病毒，源自 A/Aichi/2/68 (H3N2) 和 B/Yamagata 之间的杂交。在 MDCK 细胞中，B/Yamagata 在 39.5°C 下的铺板效率低于 10−3，而克隆 6-10 或 A/Aichi 的铺板效率高于 10−1。从混合产量中选择的爱知血清型 HA 的 15 个克隆中，有 4 个对温度敏感（ts），其中 B 型病毒优于 A 型病毒，39.5°C 的铺板效率小于 10−2，超过了克隆 6-10 后代中自发突变的频率。因此，共存的 B 型病毒不仅干扰 A 型病毒的复制，而且使其对温度敏感。使用流感病毒 A/WSN (H0N1) 的一组突变体对 4ts 克隆进行基因分析表明，与仅 NP 基因缺陷的克隆 6-10 的自发突变体相比，这些克隆具有多个基因的损伤，包括 m 中的公共缺陷。 聚丙烯酰胺凝胶这些克隆的病毒RNA和蛋白质的电泳显示出与克隆6-10相同的凝胶模式，尽管单个病毒多肽的合成速率因克隆而异。
SummaryA genetic cross was performed between influenza viruses B/Yamagata/1/73 and clone 6–10, an A type influenza virus derived from a cross between A/Aichi/2/68 (H3N2) and B/Yamagata. Efficiency of plating of B/Yamagata at 39.5° C was less than 10−3 in MDCK cells, while that of clone 6–10 or A/Aichi was higher than 10−1. Four of the 15 clones selected for HA of Aichi serotype from the mixed yield, where type B virus was predominant over type A, were temperature-sensitive(ts), with efficiency of plating at 39.5° C less than 10−2, exceeding the frequency of spontaneousts mutants among clone 6–10 progeny. Thus, co-existing type B virus not only interfered with the replication of type A, but also rendered it temperature-sensitive.Genetic analysis of the 4ts clones using a set ofts mutants of influenza virus A/WSN (H0N1) revealed that these clones, in contrast with the spontaneousts mutant of clone 6–10, withts defect only in NP gene, possessedts lesions in multiple genes including a commonts defect inm.Polyacrylamide gel electrophoresis of viral RNA and proteins of these clones showed an identical gel pattern to that of clone 6–10, although the rate of synthesis of individual viral polypeptide was variable from clone to clone.