The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp paratuberculosis isolated from tissues and faeces
The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp paratuberculosis isolated from tissues and faeces
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DOI：
10.1016/s0378-1135(03)00181-0
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发表时间：
2003-09-24
影响因子：
3.3
通讯作者：
Whittington, RJ
中科院分区：
文献类型：
--
作者：
Reddacliff, LA;Vadali, A;Whittington, RJ
研究了不同去污方法对绵羊分枝杆菌感染的影响。通过在去污过程中的不同点掺入组织和粪便来评估从临床样品中分离出的BACTEC培养物中的副结核菌。实验室的常规方案显示，粪便和组织中每份样本分离的微生物数量分别减少约2.7 log（10）和3.1 log（10）。这些发现对于解释阴性培养结果很重要，并且可能在流行病学研究中有用。在组织方案中增加离心步骤使回收率增加约1个对数，但导致BACTEC培养物的污染增加。这些研究也可能有助于今后改进净化程序。(C)2003 Elsevier B.V.保留所有权利。
The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated in BACTEC cultures from clinical samples was assessed by spiking tissues and faeces at various points during the decontamination procedure. Routine protocols in the laboratory were shown to decrease the number of organisms isolated per sample by about 2.7 log(10) and 3.1 log(10) for faeces and tissues, respectively. These findings are important for the interpretation of negative culture results and may be useful in epidemiological studies. Addition of a centrifugation step to the tissue protocol increased the recovery by about 1 log, but resulted in increased contamination of BACTEC cultures. These studies may also facilitate future improvements to decontamination procedures. (C) 2003 Elsevier B.V. All rights reserved.