Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease
Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease
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DOI：
10.1515/bc.2005.090
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发表时间：
2005-08-01
影响因子：
3.7
通讯作者：
Schultz, M
中科院分区：
文献类型：
--
作者：
Schmidt-Schultz, TH;Schultz, M
我们首次提取、溶解并鉴定了生长因子，如胰岛素生长因子II （IGF-II）、骨形态发生蛋白-2 （BMP-2）和转化生长因子- β (tgf - β)，这些生长因子可追溯到新石器时代（PPNB晚期）和中世纪早期的考古致密人骨和牙本质。这些因素是典型的特殊生理或病理情况下的骨代谢。采用二维电泳分离PPNB晚期和中世纪早期个体骨和牙齿的细胞外基质蛋白，银染色检测到300多个不同的蛋白点。来自同一个体（中世纪早期）的致密骨和牙齿的基质蛋白模式差异很大，只有16%的蛋白质斑点在致密骨和牙本质中都被检测到。
For the first time we have extracted, solubilized and identified growth factors, such as insulin growth factor II (IGF-II), bone morphogenetic protein-2 (BMP-2), and transforming growth factor-beta (TGF-beta), from archaeological compact human bone and tooth dentin dating from the late pre-ceramic pottery Neolithic (late PPNB) and the early Middle Ages. These factors are typical of special physiological or pathological situations in the metabolism of bone. The extracellular matrix proteins from bone and teeth of individuals from the late PPNB and early Middle Ages were separated by 2-D electrophoresis and more than 300 different protein spots were detected by silver staining. The matrix protein patterns of compact bone and tooth from the same individual (early Middle Ages) are very different and only 16% of the protein spots were detected in both compact bone and tooth dentin.