Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells
Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells
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一氧化氮合成内源性抑制剂的积累和再生内皮细胞中L-精氨酸含量的降低
DOI：
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发表时间：
1995
影响因子：
7.3
通讯作者：
S. Obayashi
中科院分区：
文献类型：
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作者：
H. Azuma;J. Sato;H. Hamasaki;A. Sugimoto;E. Isotani;S. Obayashi
1我们检测了兔颈动脉球囊剥脱后6周内皮细胞（EC）的再生、新生内膜形成、内皮依赖性舒张（EDR）降低以及再生EC中L-精氨酸、NG-单甲基-L-精氨酸（l-NMMA）、不对称NG、NG-二甲基精氨酸（ADMA）和对称NG、NG-二甲基精氨酸（SDMA）含量的变化。2内皮细胞在6周内完全再生，内膜增生明显，EDR降低。3再生EC中的L-NMMA和ADMA含量（分别为23.5 ± 4.3和21.2 ± 2.0 pmol mg−1 DNA），与对照组相比，差异有统计学意义（P<0.05和P<0.01）（8.8 ± 3.0）和（7.4 ± 3.0）; 1.9 pmol mg−1 DNA），而L-精氨酸显著与对照EC（47，870 ± 1，890 pmol mg − 1 DNA）相比，再生EC（31，470 ± 1，050 pmol mg − 1 DNA）中的DNA含量降低（P< 0.005）。SDMA含量低于测定限度。4 L-NMMA和ADMA以浓度依赖性方式抑制乙酰胆碱诱导的EDR，而SDMA则无此作用。添加L-精氨酸可防止L-NMMA和ADMA的抑制作用，但D-精氨酸不能。5这些结果表明，一氧化氮合成的内源性抑制剂的积累和L-精氨酸含量的降低与再生EC和新生内膜形成的NO产生/释放减少有关。
1 We examined regeneration of endothelial cells (ECs), neointima formation, decreased endothelium‐dependent relaxation (EDR) and changes in the contents of L‐arginine, NG–monomethyl‐L‐arginine (l‐NMMA), asymmetrical NG, NG–dimethylarginine (ADMA) and symmetrical NG, NG–dimethylarginine (SDMA) in the regnerated ECs, 6 weeks after balloon denudation of the rabbit carotid artery. 2 Regeneration of ECs was completed in 6 weeks and a significant neointima formation accompanied by the decreased EDR was observed. 3 L‐NMMA and ADMA contents in the regenerated ECs (23.5 ± 4.3 and 21.2 ± 2.0 pmol mg−1 DNA, respectively) were significantly (P<0.05 and P<0.01) higher than those in the control ECs (8.8 ± 3.0 and 7.4plusmn;1.9 pmol mg−1 DNA, respectively), whereas L‐arginine was significantly (P< 0.005) decreased in the regenerated ECs (31,470±1,050 pmol mg−1 DNA) as compared to that in the control ECs (47,870±1,890 pmol mg−1 DNA). SDMA content was below the assay limits. 4 L‐NMMA and ADMA, but not SDMA, inhibited the EDR induced by acetylcholine in a concentration‐dependent manner. The inhibition with L‐NMMA and ADMA was prevented by an addition of L‐arginine, but not by D‐arginine. 5 These results suggest that the accumulation of endogenous inhibitors for nitric oxide synthesis and decreased L‐arginine content are associated with decreased NO production/release from regenerated ECs and neointima formation.
DOI：
10.1161/01.atv.1.2.107
发表时间：
1981-03
期刊：
Arteriosclerosis
影响因子：
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作者：
S. Schwartz;C. Gajdusek;S. Selden
通讯作者：
S. Schwartz;C. Gajdusek;S. Selden