Role of plants in the transmission of Asaia sp., which potentially inhibit the Plasmodium sporogenic cycle in Anopheles mosquitoes
Role of plants in the transmission of Asaia sp., which potentially inhibit the Plasmodium sporogenic cycle in Anopheles mosquitoes
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DOI：
10.1038/s41598-020-64163-5
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发表时间：
2020-04-28
影响因子：
4.6
通讯作者：
Mediannikov, Oleg
中科院分区：
文献类型：
--
作者：
Bassene, Hubert;Niang, El Hadji Amadou;Mediannikov, Oleg
目前，针对疟疾及其传播的生物控制是一个巨大的挑战。与植物相关的 Asaia 属细菌经常在食蜜节肢动物中发现，它们被认为对蚊子体内疟原虫的发育具有天然的间接作用。然而，人们对其自然循环几乎一无所知。在这里，我们展示了产花蜜植物在 Asaia 的宿主和传播中的作用。我们从塞内加尔的野生蚊子和花朵中分离出 Asaia 菌株，并证明细菌从受感染的蚊子传播到不育的花朵，然后通过花蜜喂养传播到 26.6% 的未受感染的蚊子。因此，产生花蜜的植物可能会自然地获得按蚊，然后通过食源性污染在按蚊上定殖。最后，Asaia 可能在自然环境中减少按蚊的媒介能力方面发挥间接作用（由于 Asaia 的疟原虫拮抗能力），并可用于开发基于 Asaia 的副转基因疟疾控制工具。
Biological control against malaria and its transmission is currently a considerable challenge. Plant-associated bacteria of the genus Asaia are frequently found in nectarivorous arthropods, they thought to have a natural indirect action on the development of plasmodium in mosquitoes. However, virtually nothing is known about its natural cycle. Here, we show the role of nectar-producing plants in the hosting and dissemination of Asaia. We isolated Asaia strains from wild mosquitoes and flowers in Senegal and demonstrated the transmission of the bacteria from infected mosquitoes to sterile flowers and then to 26.6% of noninfected mosquitoes through nectar feeding. Thus, nectar-producing plants may naturally acquire Asaia and then colonize Anopheles mosquitoes through food-borne contamination. Finally, Asaia may play an indirect role in the reduction in the vectorial capacity of Anopheles mosquitoes in a natural environment (due to Plasmodium-antagonistic capacities of Asaia) and be used in the development of tools for Asaia-based paratransgenetic malaria control.