Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time.
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time.
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DOI：
10.3390/bios5020199
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发表时间：
2015-04-16
期刊：
影响因子：
--
通讯作者：
Graham ES
中科院分区：
文献类型：
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作者：
Kho D;MacDonald C;Johnson R;Unsworth CP;O'Carroll SJ;du Mez E;Angel CE;Graham ES
xCELLigence技术是一种实时细胞生物传感器，可测量细胞对印刷在定制设计的E板上的高密度金电极阵列的净粘附力。细胞粘附的强度受多种因素影响，包括细胞类型、细胞活力、生长、迁移、扩散和增殖。因此，我们假设xCELLigence生物传感器技术将为在众多不同的实验、临床或药理学背景下测量药物反应提供有价值的平台。在本文中，我们展示了xCELLigence技术如何在识别（1）细胞是否对特定药物产生反应，以及（2）药物反应窗口方面发挥了宝贵的作用。后一个方面通常留给经典终点测定中的有根据的猜测工作，而生物传感器技术揭示了真实的时间响应的时间曲线，这使得能够在同一测定中对急性响应和长期响应进行分析。根据我们的经验，xCELLigence生物传感器技术适用于高度靶向的药物评估，也适用于低到中等通量的药物筛选，它可以在真实的时间内产生高含量的时间数据。
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.