Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA
Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA
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DOI：
10.1007/s00438-001-0606-9
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发表时间：
2002-01-01
影响因子：
3.1
通讯作者：
Tsunewaki, K
中科院分区：
文献类型：
--
作者：
Ogihara, Y;Isono, K;Tsunewaki, K
通过与水稻和玉米叶绿体基因组全序列的比较，阐明了小麦叶绿体基因组的结构特征。小麦质体由一个134，545-bp的环状分子和20，703-bp的反向重复序列组成，其基因含量与水稻和玉米质体相同。然而，即使在基因的编码区也发现了一些结构差异。这些改变是由于两个短的直接重复序列之间的非法重组和/或复制滑动。三种谷物叶绿体DNA的总体比较表明存在一些长度突变的热点区域。而区域与集群的tRNA基因和下游的rbcL表现出分歧，在一个物种特异性的方式，在反向重复序列区域和反向重复序列和小的单拷贝区域之间的边界的ORF的缺失模式支持的概念，小麦和水稻更密切相关的仙人掌比玉米。
Structural features of the wheat plastome were clarified by comparison of the complete sequence of wheat chloroplast DNA with those of rice and maize chloroplast genomes. The wheat plastome consists or a 134,545-bp circular molecule with 20,703-bp inverted repeats and the same gene content as the rice and maize plastomes. However, some structural divergence was found even in the coding regions of genes. These alterations are due to illegitimate recombination between two short direct repeats and/or replication slippage. Overall comparison of chloroplast DNAs among the three cereals indicated the presence of some hot-spot regions for length mutations. Whereas the region with clustered tRNA genes and that downstream of rbcL showed divergence in a species-specific manner, the deletion patterns of ORFs in the inverted-repeat regions and the borders between the inverted repeats and the small single-copy region support the notion that wheat and rice are related more closely to cacti other than to maize.