Cervical cancer and microchimerism
Cervical cancer and microchimerism
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DOI：
10.1016/s0029-7844(03)00615-x
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发表时间：
2003-10-01
影响因子：
7.2
通讯作者：
Johnson, KL
中科院分区：
文献类型：
--
作者：
Cha, D;Khosrotehrani, K;Johnson, KL
目的：确定微嵌合现象是否与宫颈癌的发病机制或进展有关。方法：从八名至少有一个活产儿子并在诊断为宫颈癌后接受根治性子宫切除术的妇女中获取宫颈组织。对照组织取自四名未患有宫颈癌且至少有一名活产儿子的女性，以及三名患有宫颈癌但没有生育男孩的女性。通过荧光原位杂交分析组织切片中是否存在由 X 和 Y 染色体定义的胎儿细胞。使用免疫标记来确定假定的胎儿细胞的表型。 结果：在对大切片（约 1.5 X 2 厘米）进行分析的所有四名患者的宫颈组织中均发现了雄性细胞。在对小活检标本（约 0.1 X 0.5 厘米）进行分析的 4 名患者中，仅在 2 名患者中发现了 1 个雄性细胞。在对照组的组织标本中，无论是小切片还是大切片，都没有发现雄性细胞。在免疫标记研究中，一名患者的 18 个男性细胞中有 8 个呈 CD45 阳性，两名患者的 37 个男性细胞中有 9 个呈细胞角蛋白阳性。没有细胞对这两种标志物均呈阳性。结论：宫颈癌可能与微嵌合现象有关，微嵌合现象可能来自母胎细胞贩运。这些结果进一步扩大了微嵌合现象与女性疾病之间的潜在关系。 (C) 2003 年，美国妇产科医师学会。
OBJECTIVE: To determine whether microchimerism is involved in the pathogenesis or progression of cervical cancer.METHODS: Cervical tissue was obtained from eight women who had at least one live-born son and who underwent radical hysterectomy after a diagnosis of cervical cancer. Control tissue was obtained from four women without cervical cancer who had at least one live-born son and from three women with cervical cancer and no male births. Tissue sections were analyzed with fluorescence in situ hybridization for the presence of fetal cells, defined by an X and Y chromosome. Immunolabeling was used to determine the phenotype of the presumed fetal cells.RESULTS: Male cells were found in cervical tissue from all four patients for whom large sections (approximately 1.5 X 2 cm) were analyzed. Only one male cell was found in two of the four patients for whom small biopsy specimens (approximately 0.1 X 0.5 cm) were analyzed. No male cells were found in tissue specimens from controls, whether they were small or large sections. In immunolabeling studies, eight of 18 male cells from one patient were CD45-posiltive and nine of 37 male cells from two patients were cytokeratin-positive. No cells were positive for both markers.CONCLUSION: Cervical cancer might be associated with microchimerism, possibly from fetomaternal cell trafficking. These results further expand the potential relationship between microchimerism and disease in women. (C) 2003 by The American College of Obstetricians and Gynecologists.